Neurospora crassa arrayed knockout mutant plates

As of 2015, complete sets of arrayed mutants are not available. The FGSC does not have grant support, nor is there revenue potential to re-generate these sets. Replica plating with available resources has not proven to be reliable with cross-contamination very common. If you already paid for a full set and obtained plates before the whole set was arrayed, you may obtain the plates remaining by paying for shipping

Master Plate data sheet - Excel - plates 1 -136,  2/2/2017. Full strain lists are available below

Important notes about the arrayed strains:

Some strains are in the plates multiple times. Please see this list

Please validate strains by PCR prior to publication. If you have any concern about strains from the arrayed set, please ask for the original mutant.

If you use a Neurospora knockout strain from the functional genomics program, you should cite :

Colot HV, Park G, Turner GE, Ringelberg C, Crew CM, Litvinkova L, Weiss RL, Borkovich KA, Dunlap JC.
A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors.
Proc Natl Acad Sci U S A. 2006 Jul 5;103(27):10352-7.  Also, you may acknowledge  use of materials from the
Neurospora Functional Genomics grant P01GM68087

There is a known issue where soft phenotypes are present in some mutants and these soft phenotypes segregate separately from the deletion cassette. Available data listing these strains is online here

The following strains were recalled by the Riverside lab as being incorrect according to Southern Hybridization:

Additional strains that are of questionable authenticity.

Some plates span more than 96 strains either because there are missing strains, or some
strains have been segregated because they do not grow without special attention.

Strains that have not been placed in the main array series because they have morphological
defects will be segregated onto a Morphological mutant plate (eg, plate 29)

We prefer not to break up the arrayed sets and have made sets of strains that represent groups of
mutants.  Please do not ask for individual plates from the main series.
 

Special function plates (All of these strains are also in the main array series. )

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7/23/13