Candida at the FGSC

Knockout set from Oliver Homann
January 2010

Deletion strains were created for 165 different transcriptional regulators, and in most cases two independently derived knockouts were created for each transcriptional regulator.
They are designated 'X' and 'Y' (e.g. TF021-X and TF021-Y).

In total 4 plates are available

A description of the deletions

A spreadsheet of listing the plate contents and primers used in strain construction

The deletions are also described in Homann OR et al. A phenotypic profile of the Candida albicans regulatory network. PLoS Genet. 2009 Dec;5(12)e1000783

Knockout set from Suzanne Noble
October 2010

FGSC Plate Numbers

#             Contents
1             M1 1-121
2             M1 122-276
3             M1 277 - 501
4              M1 504-667
5              M1 669-829
6             M1 831-1009
7             M1 1010 - 1148
8             M1 1149-1152

#             Contents
9             M2 1 - 121
10          M2 122- 276
11          M2 277-501
12          M2 504- 667
13          M2 669- 829
14          M2 831- 1009
15          M2 1010- 1148
16          M2 1149- 1152

S. Noble Spreadsheet

Noble SM, French S, Kohn LA, Chen V, Johnson AD.
Systematic screens of a Candida albicans homozygous deletion library decouple morphogenetic switching and pathogenicity. Nat Genet. 2010 Jul;42(7):590-8.

Because the Mitchell Candida sets came to us piecemeal, we can send individual plates However, we do not break up sets of the Noble, Homann, or Mitchell Kinase mutants and clients who wish to receive individual strains should request them by plate and well.

Clients at for-profit entities or who wish to use these materials for profit-making purposes should contact the original depositor for access to these materials.

The fee is $100 per plate. Waivers are not available for these materials.

Shipping and Fee information

Candida strains are sent as "UN3373/ Biological Substance Category B/6.6" and are accompanied by this shipping letter. We also use a "Commercial Invoice" (click here for a sample), and a "Declaration of Biological Shipments" (click here for a sample)

Please contact the FGSC if you have resources that you would like the FGSC to consider.

The Candida albicans over-expression libraries described in the Chauvel et al PLOS ONE paper in Sept, 2012 (9:e45912) were never deposited into the FGSC collection.
The negotiation to deposit these plates is now being conducted between Kansas State and the Institut Pasteur

Information regarding the plates from the Mitchell lab

A marker-matched control is present in most of the plates. It is called strain DAY286

Descriptions of the mutants are available in the following articles.

Large-scale gene disruption using the UAU1 cassette.
Nobile CJ, Mitchell AP. Methods Mol Biol. 2009;499:175-94.

Regulation of the Candida albicans cell wall damage response by transcription factor Sko1 and PAS kinase Psk1.
Rauceo JM, Blankenship JR, Fanning S, Hamaker JJ, Deneault JS, Smith FJ, Nantel A, Mitchell AP. Mol Biol Cell. 2008 Jul;19(7):2741-51. PMID: 18434592

Requirement for Candida albicans Sun41 in biofilm formation and virulence.
Norice CT, Smith FJ Jr, Solis N, Filler SG, Mitchell AP. Eukaryot Cell. 2007 Nov;6(11):2046-55. Epub 2007 Sep 14. PMID: 17873081

Regulation of cell-surface genes and biofilm formation by the C. albicans transcription factor Bcr1p.
Nobile CJ, Mitchell AP. Curr Biol. 2005 Jun 21;15(12):1150-5. PMID: 15964282

Control of the C. albicans cell wall damage response by transcriptional regulator Cas5.
Bruno VM, Kalachikov S, Subaran R, Nobile CJ, Kyratsous C, Mitchell AP. PLoS Pathog. 2006 Mar;2(3):e21.

Candida deletion mutants from Aaron Mitchell were supported by NIH grant R01 AI057804 "Large-scale analysis of Candida albicans gene function"