Cryptococcus at the FGSC
Wild type strains
Please note that the FGSC cannot break up sets of arrayed mutants. You should request an entire set as described below, or request individual clones (please send plate and well location).
2016 Bahn set of 5 plates (MS Excel 2003)Please see Lee, Kyung-Tae, et al. "Systematic functional analysis of kinases in the fungal pathogen Cryptococcus neoformans." Nature Communications 7 (2016).
2015 Bahn set of 4 plates (MS Excel 2003)Please see Jung KW, Yang DH, Maeng S, Lee KT, So YS, et al. (2015) Systematic functional profiling of transcription factor networks in Cryptococcus neoformans. Nat Comms 6: 6757.
2016 Madhani plates (MS Excel 2003)
The new 2016 set was received at the FGSC early in July 2016 and is available as complete set of 20 plates or individual clones.
2015 Madhani plates (MS Excel 2003)
The new 2015 set was received at the FGSC early in March 2015 and is available as complete set of 22 plates or individual clones.
Regarding the 2015 sets, the UCSF team has asked that we post the following statement about acknowledgement of the resource:|
These knockout strains have been made freely available ahead of publication to the scientific community. It is requested that NIH funding (R01AI100272) for this project and the Madhani laboratory be acknowledged in any publication that uses this resource. Individuals interested in performing systematic, high-throughput analysis are asked to contact the Madhani lab to ensure coordination with ongoing efforts (email@example.com).
All of the 2015 deletion mutants are nourseothricin (NAT) resistant and can be propagated on YPD (or any other standard yeast media such as YNB or Sabouraud) supplemented with 0.1mg/ml NAT. We routinely use 0.075mg/ml NAT as we have found that specific activity of NAT varies from batch to batch. The parental strain KN99alpha is derived from H99 clinical isolate of C. neoformans Serotype A grubii and is a prototroph.
Chun and Madhani. 2010. Methods in Enzymology 470: p797 (PMID 20946836) has more information on how the knockout constructs are generated and how to propagate the strains.
In the figure, primer "1" corresponds to W1 in the spreadsheet , "2" to W2 etc.
2008 Madhani, set of 14 plates
2007 J. Lodge: Strains at the FGSC in 2 plates (A and B),
Deletion mutants are distributed as frozen
cultures in 96- well plates or on agar solidified medium (additional fees apply).
We ask $100 per plate plus shipping. International destinations require special clearance and pre-approval.
Not all strains listed in background data were sent to the FGSC.
Go to an on-line order form