Neurospora Newsletter articles describing specific research methodology

The numbers after each entry refer to articles in the Neurospora Newsletter
The listing of these articles can be found here.
We are putting the old newsletters online in reverse chronological order. You will find them here

Biochemical Methods

acetone powder preparation 389
amino acid pool, extraction 144, 208, 628
antigenic screening of isozymes 204
carotenoids, induction 570
chromatographic effluent, concentration 592
cytochromes, isolation 66

electrophoresis

acrylamide gel 567
cellulose acetate 215
extract from single perithecium 401
gel electrophoresis of amylases 178
polyacrylamide gel electrophor. 506
separation of isozymes 334

glucose-6-phosphate, assay of

steady-state level 82

mycelial suspensions, rapid sampling 424

nuclear density, measurement 578

permeabilizing hyphae 228

phosphorus, total: measurement 243, 321

proteins

concentration - dilute solutions 258
extraction 54, 189, 208, 567
localization in eluates 192
staining 185

radioactive extracts from perithecia 287

translation, cell-free system 155

Cell Fractionation

cell breakage 134
cell walls, isolation 351
mitochondria, isolation 66, 218, 231, 324
nuclei, isolation 159, 242, 319, 578
organelles, mycelial 625
ribosomes, isolation 593
subcellular fractions, isolation 390

Conidia

counting 209
disruption 296
harvest from solid media 89
induction 221
microconidial strains, estimating number
of multinucleate conidia 59
permeabilizing 529
protein, measurement 124, 525

Cytology

in vitro observation of somatic
cell division 612
staining nuclei 275, 348, 484, 613
preparing asci for EM 52

Enzymes

assay

acetyl CoA synthetase 188
adenylosuccinate lyase 579
anthranilate synthetase 99
arginase 381
arginosuccinate synthetase 610
aspartate transcarbamylase 140, 249
carbamyl phosphate synthetase 494
carbamylphosphokinase 140
cellulase 175
citrate synthase
fructose diphosphatase 355, 490
fumarate hydratase 188
ß-galactosidase 507
glucose-6-P-dehydrogenase 591
ß-glucosidase 507
glutamate dehydrogenase (NADP) 297
glutamate dehydrogenase (NAD) 297
glutamic acid decarboxylase 228
glutamine synthetase 297
hexokinase 311
isocitrate dehydrogenase (NAD) 188
isocitrate dehydrogenase (NADP) 188
isocitrate lyase 188
kynureninase 608
kynurenine-3-hydroxylase 98
malate dehydrogenase 188, 390
malate synthase 188
nucleosidase 354
ornithine transcarbamylase 140, 249
phosphoenolpyruvate carboxykinase 188
phosphofructokinase 294, 590
polyphosphatase 344
proteolytic enzymes 158, 493
pyruvate carboxylase 61
pyruvate kinase 295, 297
tyrosinase 199

crystallization 276

extraction

adenylosuccinate lyase 579
anthranilate synthetase 99
arginosuccinate synthetase 580
aspartate transcarbamylase 140
carbamylphosphokinase 140
exoenzymes from Trichderma viride 2
fructose diphosphatase 355, 4490
ß-galactosidase 283, 507
glucamylase (gamma-amylase) 177
glucose-6-P dehydrogenase 24, 122, 591
ß-glucosidase 507
glutamic acid decarboxylase 228
glutamine synthetase 298
hexokinase 311
histidinol dehydrogenase 135
isocitrate lyase-2 501
myo-inositol-1-P-synthase 24, 638
ornithine transcarbamylase 140
phosphofructokinase 294
pyruvate carboxylase 61
pyruvate kinase 295

immunological methods 638

induction

tyrosinase 244, 424

permeabilizing hyphae 228

Genetic Methods

aberrations

characterization of rearrangements 431

aneuploid production 553

ascogonia, induction 72

ascospores

collection of shot asci 408, 432
counting ascospores 363
germination improvement 566
random isolation 470
tetrad dissection 471

ascus development, testing compounds affecting 85

crossing

improving fertility

in crosses involving lys-5 5
in interallelic crosses 266, 327

inositolless death technique 499

large numbers of strains 465

mating type tests 182

perithecia

counting 154
producing large numbers 288
synchronous production 254

procedures 330, 396

protoperithecia, isolation 290

heterokaryons

het tests in liquid media 150
formation 450, 560
obtaining het compatible isolates 3
strain preservation 173, 450

mapping and linkage

analysis of progeny from interallelic crosses 326
characterization of rearrangements 431
detection of linked suppressors
multiple interchange link. testers 429
RFLP mapping 373
interspecies gene transfer 371
selection of linked markers in Sordaria brevicollis 619

mutant isolation

catalase mutants 418
conidial separation mutants 542
cpd mutants 240
filtration-concentration procedures 22, 94, 633
intramural enzyme mutants 156
meiotic mutants 241
microconidiating strains in mutant hunts 5y
nitrosoguanidine mutagenesis 618

obtaining double mutants 3

in linkage group IV 9
intragenic 97

sterility mutants 71, 604
selection of temp. sens. mutants 573
tritium suicide enrichment 503
use of temp. sens. strains 83

mutation studies

cycloheximide resistance as a

forward mutation detector 58

detection of alterations via

specific revertibility 345

null mating type, in heterokaryons 450

recombination

selective plating for detecting recombination with cr 333

test for non-random disjunction 575

transformation, DNA mediated 142, 383, 420, 518, 521, 539, 577, 582, 629

Growth methods

[abn-1] and [abn-2] 45
circadian clock studies 462
conidia, mycelia from same medium 31
continuous flow system 193
glycerol minimal medium 117
hyphal production 77
large scale culture 190, 205, 208
logarithmic growth measurement 127
nutrient-limited growth 191
obtaining mycelial pads 630
scoring [mi-1] 44
shake culture 143
slime 136, 403, 532, 627
Sordaria culture methods 183
Stanford Lab 396
Trichoderma viride 2
use of washing machines 190
Vogel's medium, modified for crossing 511

Nucleic Acids

colony hybridization technique 419

DNA

incorporation of P32 347, 492
isolation 121, 180, 372, 405, 563, 607
measurement of synthesis 212
nick-translating 320
gene library construction 181, 383

RNA

isolation 121, 561

transformation, DNA mediated 142, 383, 420, 521, 539, 577, 582, 629

Preservation of strains

freezing

-20°C 442
-70°C 535

heterokaryons 173, 450

liquid nitrogen 136, 291, 614, 621

Na2SO4 385

silica gel 81, 106, 315, 416, 417, 447, 448, 552, 621

slime 136, 291, 532, 535, 621

sucrose solution 337

Protoplasts

Polyoxin B, use in preparation 538
production from mycelia 2, 246, 519
production from os-1 mutant 537, 538

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