24 h developmental cDNA library from Aspergillus nidulans constructed by R.Aramayo
It was deposited to the FGSC in 1995 and maintained as phage lysate in SM at -80 with one freeze-thaw cycle to aliquot samples.
The developmental poly(A)+ mRNA used to construct this library was prepared as follows: conidia from the strain FGSC A26 (biA1) were germinated in a liquid medium and allowed to grow 18 h. Mycelia was harvested on top of filter paper and placed onto appropriately supplemented minimal medium. Induced mycelia were then allowed to develop for 24 h prior to harvest and mRNA extraction. Poly(A)+ mRNA was prepared using standard procedures. The cDNA library was constructed using the lambda ZAPcDNA TM+ kit lot # UC105. The primary library contained approximately 100,000 clones. After plating this primary library, the phage were harvested and stored at -70øC as suggested by Maniatis. This library has not been amplified. Genes expressed as early as 10 h have been cloned from this library.
All of them were deposited at the FGSC more than ten years ago.
pWE15 and pLORIST2 genomic cosmid libraries
The FGSC distributed these cosmid libraries (30 microtiter plates each) either separately, or more typically, together. Together, the two libraries give good coverage of the A. nidulans genome and form the basis of the chromosome specific and minimal libraries.
a list of identified clones in the pWE15 and pLORIST2 libraries
Chromosome specific libraries
All clones from the pWE15 and pLORIST2 libraries showing hybridization to chromosome-specific probes were reisolated to microtiter plates according their chromosome linkage (Brody et al. 1991. Nucleic Acids Res.19:3105-3109). A total of 38 chromosome specific plates resulted. These were sent as a complete set or as individual chromosome subsets.
Minimal Compressed library from
The minimal, ordered, compressed libraries of A. nidulans are described in Prade et al. PNAS 94:14564-14569 (1997). These libraries were picked from the pWE15 and pLORIST2 sets at the FGSC and were distributed in 16 96 well microtitre plates.
This spreadsheet lists the well identities.
A. nidulans libraries
Primers for sequencing inserts in these AMA vectors directly
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