Plasmid and Lambda Vectors

The pCB family of transformation vectors described by Sweigard et al in FGN 44 are available from the FGSC.

Gene: AMA pyrG

Plasmid Name: ANEp2
Size in KB: 14.2
Resistance: amp
Reference: Plasmid 53:191-204
Notes: for protein manipulation in A. niger

Gene: AMA pyrG

Plasmid Name: ANEp4
Size in KB: 13.8
Resistance: amp
Reference: Plasmid 53:191-204
Notes: for protein manipulation in A. niger

Gene: AMA pyrG

Plasmid Name: ANEp6
Size in KB: 13.9
Resistance: amp
Reference: Plasmid 53:191-204
Notes: for protein manipulation in A. niger

Gene: AMA pyrG

Plasmid Name: ANEp7
Size in KB: 10.8
Resistance: amp
Reference: Plasmid 53:191-204
Notes: for protein manipulation in A. niger

Gene: AMA pyrG

Plasmid Name: ANEp8
Size in KB: 11.4
Resistance: amp
Reference: Plasmid 53:191-204
Notes: for protein manipulation in A. niger

Gene: pyrG

Plasmid Name: ANIp7
Size in KB: 5.6
Resistance: amp
Reference: Plasmid 53:191-204
Notes: for protein manipulation in A. niger

Gene: pyrG

Plasmid Name: ANIp8
Size in KB: 6.1
Resistance: amp
Reference: Plasmid 53:191-204
Notes: for protein manipulation in A. niger

Gene: hph

Plasmid name: pPK2
Size in kb: 10.77
Resistance: Kan
Depositor: S. Covert
Reference: Mycological Research 105:259-264.
Vector: pPZP-201BK
Notes: For transforming fungi using Agrobacterium

Gene: GFP

Plasmid name: pFNO3 Size in kb: 6.5
Resistance: Kan
Depositor: SO
Reference: Euk Cell 3:1359-1362
Vector:
Notes: GA5-GFP + A. fumigatus pyrG. Map and sequence from Dr. Osmani (MS Word)

Gene: S-TAG

Plasmid name: pAO81 Size in kb: 5.4
Resistance: Kan
Depositor: SO
Reference: Euk Cell 3:1359-1362
Vector:
Notes: S-TAG + A. fumigatus pyrG. Map and sequence from Dr. Osmani (MS Word)

Gene: RFP

Plasmid name: pXDRFP4 Size in kb: 6.2
Resistance: Kan
Depositor: SO
Reference: Euk Cell 3:1359-1362
Vector:
Notes: GA5-mRFP + A. fumigatus pyrG. Map and sequence from Dr. Osmani (MS Word)

Gene: hph

Plasmid name: p434
Size in kb:7.1
Resistance:amp
Depositor: DAskew
Reference: BMC Microbiology 2005, 5:1
Notes:tet expression

Gene: ble

Plasmid name: p444
Size in kb:6.4
Resistance:amp
Depositor: DAskew
Reference: BMC Microbiology 2005, 5:1
Notes:tet expression

Gene: -

Plasmid name: p474
Size in kb:4.6
Resistance:amp
Depositor: DAskew
Reference: BMC Microbiology 2005, 5:1
Notes:tet expression

Gene: ble

Plasmid name: p480
Size in kb:6.1
Resistance:amp
Depositor: DAskew
Reference: BMC Microbiology 2005, 5:1
Notes:tet expression

Gene: hph

Plasmid name: p482
Size in kb:5.7
Resistance:amp
Depositor: DAskew
Reference: BMC Microbiology 2005, 5:1
Notes:tet expression

Gene: hph

Plasmid name: p500
Size in kb:6.1
Resistance:amp
Depositor: DAskew
Reference: BMC Microbiology 2005, 5:1
Notes:tet expression

Gene: hph

Plasmid name: p502
Size in kb:6.9
Resistance:amp
Depositor: DAskew
Reference: BMC Microbiology 2005, 5:1
Notes:tet expression

Gene: -

Plasmid name: p473
Size in kb:4.9
Resistance:amp
Depositor: DAskew
Reference: BMC Microbiology 2005, 5:1
Notes:tet expression

Gene: pyr4/AMA1

Name: pRG3-AMA1-NotI
Size in kb: 10
Resistance: amp
Depositor: GSM
Reference: Genetics 155:647-656
Vector: puc19
Notes: for genomic library construction

Gene: niaD/AMA1

Name: pRG3-AMA1-niaD
Size in kb: 11
Resistance: amp
Depositor: GSM
Reference: FGB 2001
Vector: puc19
Notes: for genomic library construction

Gene: niiA/AMA1

Name: pRG3-AMA1-niiA
Size in kb: 11
Resistance: amp
Depositor: GSM
Reference: FGB 2001
Vector: puc19
Notes: for genomic library construction

Gene: pyr4

Plasmid name: AMA-NotI
Size in kb:10 Kb
Resistance:Amp
Depositor: G.S. May
Reference: Genetics 155:647-656
Vector: pUC19
Notes:AMA1

Gene:pyr4

Plasmid name: AMA-niiA
Size in kb: 11 kb
Resistance: Amp
Depositor: G.S. May
Reference:FGB 31 1- 8
Vector: pUC19
Notes:AMA1

Gene:pyr4

Plasmid name: AMA-niaD
Size in kb: 11 kb
Resistance: Amp
Depositor: G.S. May
Reference:FGB 31 1- 8
Vector: pUC19
Notes:AMA1

Gene: pyrG

Plasmid name: (lambda)pAn
Size in kb: 4.0
Resistance: amp
Depositor: G.S. May
Reference: Holt and May 1993 Gene 133:95-97
Vector: pBR322
Notes: Lambda vector allows subcloning of insert pBX via the Cre-loxP site-specific recombination system
Click here for a description of the lambda pAn vector

Gene: Vector

Plasmid name: pBARGRG1
Size in kb: 6.35
Resistance: amp
Depositor: M.L. Pall
Reference: Pall and Brunelli 1994 FGN 41:63-65
Vector: pUC
Notes: contains trpC term, grg-1 (ccg-1) prom, bar and ampr grown in DH5a
Click here for a description and map of pBARGRG-1

Gene: Vector

Plasmid name: pBARMTE1
Size in kb: 5.0
Resistance: amp
Depositor: M.L. Pall
Reference: Pall 1993 FGN 40:59-62
Vector: pUC
Notes: contains trpC term, mtr prom, bar and amp^r grown in DH5a
Click here for a map and description of pBARMTE1

Gene: Vector

Plasmid name: pBARGEM7-2
Size in kb: 4.9
Resistance: amp
Depositor: M.L. Pall
Reference: Pall 1993 FGN 40:59-62
Vector: pUC
Notes: grown in DH5a
Click here for a map and description of pBARGEM7-2

Gene: Vector

Plasmid name: pBARKS1
Size in kb: 4.5
Resistance: amp
Depositor: M.L. Pall
Reference: Pall 1993 FGN 40:59-62
Vector: pUC
Notes: grown in DH5a
Click here for a map and description of pBARKS1

Gene: Vector

Plasmid name: pBARGPE1
Size in kb: 5.5
Resistance: amp
Depositor: M.L. Pall
Reference: Pall 1993 FGN 40:59-62
Vector: pUC
Notes: grown in DH5a
Click here for a map and description of pBARGPE1

Gene: Vector

Plasmid name: pBARGEM5-1
Size in kb: 4.5
Resistance: amp
Depositor: M. Pall
Reference: Pall 1993 FGN 40:59-62
Vector: pUC
Notes: grown in DH5a
Click here for a map and description of pBARMGEM5-1

Gene: Vector

Plasmid name: pBARGEM7-1
Size in kb: 4.5
Resistance: amp
Depositor: M. Pall
Reference: Pall 1993 FGN 40:59-62
Vector: pUC
Notes: grown in DH5a
Click here for a map and description of pBARGEM7-1

Gene: Vector

Plasmid name: lambdaBARGPE1
Size in kb: 40.9
Resistance: amp
Depositor: M.L. Pall
Reference: Pall and Brunelli 1994 FGN 41:63-65
Vector: pUC
Notes: contains lambda arms (19.6 kb + 15.8 kb)

Gene: Vector

Plasmid name: lambdaBARMTE1
Size in kb: 40.4
Resistance: amp
Depositor: M.L. Pall
Reference: Pall and Brunelli 1994 FGN 41:63-65
Vector: pUC
Notes: contains lambda arms (19.6 kb + 15.8 kb)

Gene: Vector

Plasmid name: lambda-BARGEM7
Size in kb: 40.3
Resistance: amp
Depositor: M.L. Pall
Reference: Pall and Brunelli 1994 FGN 41:63-65
Vector: pUC
Notes: contains lambda arms (19.6 kb + 15.8 kb)
Click here for a description and map of lambda-BARGEM7

Gene: Vector

Plasmid name: lambda-IIBARGEM7B
Size in kb: 40.3
Resistance: amp
Depositor: J. P. Brunelli
Reference: Brunelli and Pall 1995 FGN 42:21-22
Vector: pBARGEM7-2
Notes: contains lambda arms (19.6 kb + 15.8 kb) yet accepts up to 18 Kb inserts
Click here for a description and map of lambda-IIBARGEM7B

Gene: Vector

Plasmid name: lambda-BARGRG1
Size in kb: 41.7
Resistance: amp
Depositor: M.L. Pall
Reference: Pall and Brunelli 1994 FGN 41:63-65
Vector: pUC
Notes: contains lambda arms (19.6 kb + 15.8 kb)

Gene: Vector

Plasmid name:pBC-hygro
Size in kb: 6.8
Resistance: Cm
Depositor: P. Silar
Reference: Silar, FGN 42:73
Vector: pBC
Notes: hph from pMOcosX under control of N. crassa cpc-1 promoter and A. nidulans trpC terminator
Click here for a map and description of pBC- hygro

Gene: Vector

Plasmid name:pBC-phleo
Size in kb: 6.1
Resistance: Cm
Depositor: P. Silar
Reference: Silar, FGN 42:73
Vector: pBC
Notes: Phleo cassette from pUT703 (Curr Genet 20:309-314) with A. nidulans gpg promoter and S. cerevisiae CYC1 terminator
Click here for a map and description of pBC- phleo

Gene: qa-2 promoter, qa-4 terminator

Plasmid name: pWF1
Size in kb: 4.3
Resistance: amp
Depositor: H. Weiss
Reference: Fecke et al 1993 Fungal Genetics Newsl 40:34
Vector: pT7T3
Notes: antisense RNA expression vector; pT7T3 from Pharmacia
Click here for a description of pWF1

Gene: hygBr

Plasmid name: pES200
Size in kb: 6.2
Resistance: amp
Depositor: C. Staben
Reference: Staben et al. 1989 Fungal Genet. Newsl. 36:79-81
Click here for a map and description of pES200

Gene: hygBr

Plasmid name: pCSN43
Size in kb: 5.3
Resistance: amp
Depositor: C. Staben
Notes: pCSN43 and pCSN44 have SalI fragment from pDH25 in reverse orientations.
Reference: Staben et al. 1989 Fungal Genet. Newsl. 36:79-81
Click here for a map and description of pCSN43  DNA sequence (MS Word format)

Gene: hygBr

Plasmid name: pCSN44
Size in kb: 5.3
Resistance: amp
Depositor: C. Staben
Reference: Staben et al. 1989 Fungal Genet. Newsl. 36:79-81
Notes: pCSN43 and pCSN44 have SalI fragment from pDH25 in reverse orientations
Click here for a map and description of pCSN44  DNA sequence (MS Word format)

Gene: hygBr

Plasmid name: pCB1003
Size in kb: 4.1
Resistance: amp
Depositor: A. Carroll
Reference: Carroll et al. 1994. Fungal Genet. Newsl. 41:22
Notes: Altered hygR cassette from pCSN43 in pUC19
Click here for a description of pCB1003

Gene: hygBr

Plasmid name: pCB1004
Size in kb: 4.4
Resistance: cap
Depositor: J. Sweigard
Reference: Carroll et al. 1994. Fungal Genet. Newsl. 41:22
Notes: Altered hygR cassette from pCSN43 in pBC SK+
Click here for a map of pCB1004

Gene: hygBr

Plasmid name: pMOcosX
Size in kb: 8.8
Resistance: amp
Depositor: M. Orbach
Click here for a map and description of pMOcosX

Gene: lacZ

Plasmid name: pDE1
Size in kb: 9.5
Resistance: amp
Depositor: Ebbole
Reference: Ebbole 1990 FGN 37:15-16
Vector: pSP72
Notes: EcoR1 - ~6.2 + 3.3
Click here to see a restriction map of pDE1

Gene: lacZ

Plasmid name: pDE2
Size in kb: 9.5
Resistance: amp
Depositor: Ebbole
Reference: Ebbole 1990 FGN 37:15-16
Vector: pSP72
Notes: see pDE1
Click here to see a restriction map of pDE2

Gene: lacZ

Plasmid name: pDE3
Size in kb: 9.5
Resistance: amp
Depositor: Ebbole
Reference: Ebbole 1990 FGN 37:15-16
Vector: pSP72
Notes: see pDE1
Click here to see a restriction map of pDE3

Gene: hyg/mtr

Plasmid name: pMTR::HYG
Size in kb: 8.1
Resistance: amp
Depositor: J. P. brunelli
Reference: Schroeder et al., 1995 FGN 42:65-68
Vector: pCVN2.9
Notes: for studying transformation
Click here to see a restriction map of pMTR::HYG

Gene: qa-2

Plasmid name: pMSN1
Size in kb: 5.8
Resistance: amp
Depositor: M.A. Nelson
Reference: Nelson and Metzenberg 1992 FGN 39:59-60
Vector: pGEM
Notes: qa-2 as a selectable marker in pGEM
Click here for a map and description of pMSN1

Gene: qa-2 promoter, trpC terminator

Plasmid name: pMYX2
Size in kb: 7.3
Resistance: amp
Depositor: C.P. Selitrennikoff
Reference: Campbell et al. 1994. FGN 41:20-21
Vector: pGEM
Notes: For expression and modification of cDNA in N. crassa
Click here for a description of pMYX2 and pMYX10

Gene: qa-2 promoter, qa-4 terminator, hygR

Plasmid name: pMYX10
Size in kb: 7.5
Resistance: amp
Depositor: C.P. Selitrennikoff
Reference: Campbell et al. 1994. FGN 41:20-21
Vector: pWF1
Notes: For expression and modification of cDNA in N. crassa
Click here for a description of pMYX2 and pMYX10

Gene: BmlR (beta-tubulin)

Plasmid name: pSV50
Size in kb: 7.9
Resistance: amp
Depositor: S. Vollmer
Reference: Vollmer and Yanofsky 1986 PNAS 83:4869-4873
Notes: Cosmid, benomyl resistant. EcoR1 - 3.5 + 4.5
Click here to see a restriction map of pSV50

Gene: hph

Plasmid name: AN26
Size in kb:7.4 kb
Resistance: amp
Depositor: J. Taylor
Reference: FGN 43:52-53
Notes: Cosmid,
Click here to see a restriction map of AN26

Gene: his-3 targeting

Plasmid name: pRAUW122
Size in kb: 14.3 kb
Resistance: amp
Depositor:
Reference: FGN 43:9-13
Vector: pGEM-9Zf(-)
Click here for a restriction map of pRAUW122

Gene: his-3 targeting

Plasmid name: pBM60
Size in kb: 6.8 kb
Resistance: amp
Depositor: B. Margolin
Reference: FGN 44: 34-36
Vector: pSP72
Click here for a restriction map of pBM60 and pBM61

Gene: his-3 targeting

Plasmid name: pBM61
Size in kb: 6.8 kb
Resistance: amp
Depositor: B. Margolin
Reference: FGN 44: 34-36
Vector: pSP72
Click here for a restriction map of pBM60 and pBM61

Gene: Tn5

Plasmid name: pLHI
Size in kb:
Resistance: Kan
Depositor: L. Hamer
Reference:Fungal Genetics Newsletter 44:19-23
Vector: pFTS
Note:Temperature sensitive- grow at 24

Gene: Tn5

Plasmid name: pLH3
Size in kb:
Resistance: Kan
Depositor: L. Hamer
Reference: Fungal Genetics Newsletter 44:19-23
Vector:pFTS
Note:Temperature sensitive- grow at 24

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Last modified 4/08 KMC