Contig Overlaps

In the Broad Institute genome, 147 contigs were linked by end-reads of fosmid or BAC clones to form 89 supercontigs. The 16 largest supercontigs are related by genetically mapped markers to the 16 chromosome arms: see Genes in contigs.

In fact, many adjacent contigs in each supercontig overlap with each other, or with previously unlocated bridging contigs. In this table contigs are listed in order of supercontig and contig number, detailing, where present, overlaps between adjacent contigs.
The orientations (+/-) of previously unlocated, inserted contigs, are shown relative to their new context.
Where overlapping contigs differ by insertion/deletion, two figures are given for the overlap length, the first for the contig listed, the second for the following contig that overlaps it.
Figures in the "Mismatches" column include unmatched terminal bases where these occur. Mismatches near contig ends are labelled "e": near contig end, or "s": near start of next contig.

Links to telomeres located by Li, W., Rehmeyer, C.J., Staben, C. & Farman, M.L. (refs 1088, 1200).

Links:
Genes in contigs, linkage maps
Chomosomal locations of autocalled genes
List of previously unlocated contigs
Maps home page


Supercontig 1, Linkage group VIII-R
Contig
length
Overlap with
next contig
Mismatches
in overlap
Comment
1
10851
-

Link to Telomere T9
2
168814
-


3
127977
536
0

230 (-)
5336
575
0

4
155056
558
0

246 (-)
3023
560
0

5
450355
513
0

186 (+)
10198
483
1e

6
266891
-


7
773302
-


8
49390
2818, 2817
3e

9
14258
-


10
175166
766
35s

11
61200
494
2s

180 (-)
11235
751
1e, 1s

12
174100
2695
2

13
249512
1605
3

14
364334
1751
0

15
178329
790, 791
18e

16
451587
1270
1e

17
347844
616
0

225 (-)
5462
601
0

18
175793
471
0

192 (-)
9030
574
0

19
71437
-


20
21570
-


21
2196




Supercontig 2, Linkage group VII-R
Contig
Length
Overlap with
next contig
Mismatches
in overlap
Comment
175 (+)
19880
728
0

22
276296
641
0

224 (-)
5623
1214, 1213
11e

23
67024
943
0

213 (-)
6639
226, 227
13e

24
43525
1205
0

25
229102
353
0

182 (+)
10620
402
0

26
389371
(10)
0
(C)10 at end contig 26, (C)27 at start contig 27 doubtful
27
113764
-


28
98898
-


29
525887
488
40s

30
89802
-


31
2490
-


32
435990
-


33
2149
1680
3

244 (+)
3100
1316, 1317
23e

34
177275
-

Gypsy-1 element, with agreeing target site duplications, forms probable bridge of 4037 nt gap.
35
84073
73, 74
29e

36
96901
709
0

241 (-)
3865
566
0

37
75346
568
23e

38
233386
997
0

242 (+)
3786
808
0

39
240080
-


220 (+)
6090
401
1e

40
150763
1169
0
AF497720 (mnpA) overlaps contig 40 by 1169 bp (0 mismatches) and contig 41 by 584 bp (4 mismatches)
41
64302
327
50e

42
99573
126, 128
21e

43
338545


Link to telomere T12

Supercontig 3, Linkage group VI-R
Contig
Length
Overlap with
next contig
Mismatches
in overlap
Comment
44
17771
-

Link to telomere T10
45
83158
656
1

235 (-)
4760
1009
0

46
103482
3366
0

47
137873
-


48
95913
894. 892
6e

227 (+)
5437
340, 341
13e, 3s

49
206454
1542
0

50
47652
904
1s

189 (-)
10019
914
3s

51
1114266
-


52
82934
1315
0

53
51172
763
0

231 (+)
5185
512
0

54
183474
1612
0

55
492661
-

Simple sequences at end contig 55 and start contig 56
56
45063
3028
128s

57
28094
1366
0

58
83422




Supercontig 4, Linkage group II-R
Contig
Length
Overlap with
next contig
Mismatches
in overlap
Comment
59
177453
740, 741
3s
Link to telomere T4
222 (+)
5781
474
0

60
68571
(-)

476 bp overlap of I-1 elements, but 52 mismatches
61
865047
577
0

191 (-)
9200
525
3

62
201480
330
4s

63
41959
-


64
203363
-


65
278071
-


66
88283
486
0

236 (-)
4673
616, 616
1e

67
254221
75
25e

68
250663
-


69
80995
-


70
41226
4410, 4407
10e

174 (+)
26679
-


71
8494




Supercontig 5, Linkage group III-L
Contig
Length
Overlap with
next contig
Mismatches
in overlap
Comment
72
17816
-


73
3923
-


74
12387
-


75
213493
-

Possible I-1 retrotransposon bridge of 3467 nt gap
76
153210
-


77
132523
359
0

179 (-)
11756
657, 658
1e, 5s

78
353456
584
0

238 (+)
4644
287, 288
33

79
209053
1847
5
Mismatches clustered in middle of overlap
80
335440
444
0

201 (-)
7389
1628
0

81
156263
698
2

183 (-)
10603
640
2s

82
44985
-


83
55015
995
2e

233 (+)
4981
953
1s

84
621812
-


85
34531
-


86
112247


Link to telomere T11

Central fragment of supercontig 6 (now 6a), Linkage group V. Displaced by finding that telomeric contig 216 overlaps contig 91, and by telomere location by the methods of Li et al. 2005 (ref. 1088). Probably flanked by centromere and nucleolus organizer
Contig
Length
Overlap with
next contig
Mismatches
in overlap
Comment
87
12990
-


88
183820
-


89
282268
-


90
3363
-



Supercontig 6b, linkage group V-R
226 (+)
5454
-

Telomere T15 at start
Contig 92 shows links to TelContig complex TC11, probably to the remaining unattached telomere: T15 on contig 226.
92
62660
4191, 4190
1

210 (-)
6735
619
0

93
340023
1500
0

94
523724
4545, 4546
1

95
103820
1223
0
4 unmatched bp at end contig 95
96
98894
1303
0

199 (+)
7686
516
0

97
24746
620
4

195 (+)
8077
825
0

98
565485




Supercontig 7, Linkage group I-L
Contig
Length
Overlap with
next contig
Mismatches
in overlap
Comment
99
2005
-


100
337251
-


101
209568
739
1e, 1s

194 (+)
8226
644
0

102
132643
1881
2e
Mismatches near end of contig 102
103
69272
1605
0

104
297769
1228
3

207 (+)
6880
754
0

105
277183
329
0

204 (+)
6994
680
0

106
67855
297
1, 5e

107
444822
15
2e

208 (-)
6838
734, 735
1e

108
352786


Link to telomere T7

Supercontig 8, linkage group I-R
Contig
Length
Overlap with
next contig
Mismatches
in overlap
Comment
109
233950
623
1s

234 (-)
4876
1211
0

110
298245
1371
0

111
62418
-


112
353724
-


113
316066
652, 653
1e

237 (-)
4650
542
0

114
25936
1657
0

187 (-)
10087
2576
0

115
127320
829, 830
2e

181 (+)
10660
2263, 2261
38s

116
56802


Link to telomere T14

Supercontig 9, Linkage group IV-L
Contig
Length
Overlap with
next contig
Mismatches
in overlap
Comment
117
274575
-

Possible I-1 retrotransposon bridge of 1050 nt gap
211 (+)
6721
711
0

118
105880



119
137987
-


120
3924
-


121
13722
-

AMA-1 sequence and MATE-1b (DNA-3_AN) transposon bridge 3398 nt gap
122
214949
649
0

223 (+)
5626
815
0

123
128436
1631
0

205 (-)
6930
530
0

124
55685
1311
0

125
32723
480
6, 30s

126
40772
-


127
138927
-


128
280307


Link to telomere T2

Supercontig 10, Linkage group IV-R
Contig
Length
Overlap with
next contig
Mismatches
in overlap
Comment
129
627835
-


130
308583
-
-

131
116788
1713
1s

132
254738
-

Gypsy-1 element with matching target site duplications forms probable bridge of 2943 bp gap
133
81213


Link to telomere T13

Supercontig 11, Linkage group II-L
Contig
Length
Overlap with
next contig
Mismatches
in overlap
Comment
134
78670
-

Telomere T6 at start
135
301041
-


136
31127
1218
0

232 (+)
5001
610
0

137
32282
654
6s

193 (+)
8744
895, 896
7e

138
39791
-

contig 138 end and contig 139 start are simple sequences
139
347029
760, 757
3e

215 (+)
6468
613
0

140
26834
618
0

203 (+)
7055
1060
0

141
203328
3978, 3977
27e, 1s

190 (+)
9210
170
0

142
43730
(-)

I-1 element putatively bridges 3262 bp gap
143
30509
507. 511
46

144
72448
670
0

229 (-)
5381
855
0

145
176570




Supercontig 12, Linkage group V-L
Contig
Length
Overlap with
next contig
Mismatches
in overlap
Comment
146
11447
-

Link to telomere T8
147
5126
38
0
Doubtful: overlap is Gypsy-1 element, but target-site duplications unmatched.
148
4102



149
38673
-


150
109819
-


151
72894
939, 440
3e

212 (+)
6710
1368, 1369
1e

152
53749
-


153
403022
789
0

196 (-)
8076
2654, 2657
3

154
34335
5053, 5052
2

155
40523
-

AJ575188 (azgA) overlaps contig 155 by 385 bp and contig 156 by 2628 bp, with 0 mismatches
156
11675
-


157
136262




Supercontig 13, Linkage group III-R
Contig
Length
Overlap with
next contig
Mismatches
in overlap
Comment
158
283337
605
0
Link to telomere T1
209 (-)
6773
661
0

159
58286
1821
0

160
146980
864, 863
7s

214 (-)
6579
621, 620
1s

161
266267
918
0

198 (+)
7688
772
0

162
65002
-

34 nt gap to contig 163 bridged by PCR fragment (ref. 1096) after deletion of 36 incorrect bases from ends of both contigs
163
91924




Supercontig 14, Linkage group VII-L
Contig
Length
Overlap with
next contig
Mismatches
in overlap
Comment
164
150254
633
0

176 (+)
15731
537
0

165
114598
2911, 2907
5, 1s

178 (+)
12319
663
0

166
41526
724, 727
5s

167
41972
-


168
229305
1586, 1585
4s

219 (+)
6367
504
0

200 (+)
7501


Telomere T5 at end

Supercontig 15, Linkage group VI-L
Contig
Length
Overlap with
next contig
Mismatches
in overlap
Comment
169
351151
473
0

206 (+)
6924
860
0

170
216503


Ends in telomere T3

Supercontig 16, Linkage group VIII-L
Contig
Length
Overlap with
next contig
Mismatches
in overlap
Comment
216(-)
6436
3852
6, 15e
Telomere T16 at start.
91
6778
-


171
24092
-


172
523550
-


173
11633



List of previously unlocated contigs
Chomosomal locations of autocalled genes
Genes in contigs and linkage maps
Maps home page