Medium supplements for mutant classification..... Other supplements
| MARKER
|
CLASS- IFICATION |
SUPPLEMENT
|
FINAL CONC. |
MEDIUM
|
STOCK SOL'N |
AMOUNT
ADDED to 200 ml |
| acr
|
Res
|
acriflavine
|
25
ug/ml
|
CM
|
5
mg/ml
|
1
ml
|
| act
|
Res
|
cylcoheximide
(Actidione)
|
1
mg/ml
|
MM,
CM
|
||
| acu
|
C-u
|
acetate
|
150
mM
|
-C
|
6
M (pH7)
|
5 ml
|
| ad
|
Rq*
|
adenine
|
0.5
mM
|
MM
|
50
mM
|
2 ml
|
| al
|
N-u
|
allantoin
|
0.1
mg/ml
|
-N
|
||
| ahr
|
Res
|
aspartic
hydroxamate
|
0.3
mM
|
MM
|
||
| amd
|
N-u,
C-u
|
acetamide
|
10
mM
|
-N
or -C
|
||
| an
|
Rq
|
aneurin
(=thiamine)
|
0.1
ug/ml
|
MM
|
0.2
mg/ml
|
100
ul
|
| ara
|
C-u
|
L-arbinose
|
1%
|
-C
|
||
| arg
|
Rq*
|
arginine
|
4
mM
|
MM
|
0.2
M
|
4 ml
|
| ben
|
Res
|
Benlate
(= 50% benomyl)
|
2
ug/ml
|
MM,
CM
|
0.2
mg/ml ethanol
|
2 ml
|
| bi
|
Rq
|
biotin
|
0.02ug/ml
|
MM
|
40
ug/ml
|
100
ul
|
| car
(=cbx)
|
Res
|
carboxin
(Vitavax)
|
12.5ug/ml
|
-C
+ 150 mM neutral acetate
|
||
| chlA
|
Res
|
chloroneb
(Demosan)
|
50
ug/ml
|
MM
|
||
| cho
|
Rq*
|
choline
|
0.2
mg/ml
|
MM
|
4
mg/ml
|
1 ml
|
| cnx
|
Rq
|
nitrite
(sodium)
|
5
mM
|
MM
|
1
M
|
1 ml
|
| "
|
Rq
|
ammonium
(tartrate or chloride)
|
5
mM
|
MM
|
1
M
|
1 ml
|
| "
|
Rq
|
other
N-source
|
5
mM
|
MM
|
||
| "
|
N-u
|
nitrate
(sodium)
|
10
MM
|
(=MM)
|
| |
| "
|
N-u
|
hypoxanthine
|
100
ug/ml
|
-N
|
| |
| "
|
Res
|
chlorate
(potassium or sodium)
|
50
mM
|
-N
+ 5 mM proline, arginine or urea
|
1
M
|
10
ml
|
| fac
|
C-u
|
acetate
|
150
mM
|
-C
|
6
M (pH7)
|
5
ml
|
| fpa
|
Res
|
p-fluorophenyl-alanine
|
0.6
mM
|
CM
|
24
mM
|
5
ml
|
| hx
|
N-u
|
hypoxanthine
|
100
ug/ml
|
-N
|
||
| gal
|
C-u
|
galactose
|
1%
|
-C
|
||
| gam
|
Res
|
molybdate
(sodium)
|
0.33
mM
|
-N
+ 5 mM NH4
|
| |
| "
|
C-u
|
galactose
|
1%
|
-C
|
||
| his
|
Rq*
|
histidine
|
4
mM
|
MM
|
0.2
M
|
4
ml
|
| hx
|
N-u
|
hypoxanthine
|
100
ug/ml
|
-N
|
||
| ile
|
Rq*
|
isoleucine
|
4
mM
|
MM
|
0.2
M
|
4
ml
|
| ima
|
Res
|
imazalil
(Plantvax)
|
2
ug/ml
|
MM
|
||
| ino
|
Rq*
|
m-inositol
|
20
ug/ml
|
MM
|
4
mg/ml
|
1
ml
|
| iod
|
Res
|
iodoacetate
|
0.4
mg/ml
|
CM
|
||
| lac
|
C-u
|
Lactose
|
1%
|
-C
|
||
| lu
|
Rq*
|
Leucine
|
4
mM
|
MM
|
0.2
M
|
4
ml
|
| lys
|
Rq*
|
Lysine
|
4
mM
|
MM
|
0.2
M
|
4
ml
|
| mal
|
C-u
|
maltose
|
1%
|
-C
|
||
| meth
|
Rq
|
methionine
|
1
mM
|
MM
|
0.2
M
|
1
ml
|
| mol
|
Res
|
molybdate
(sodium)
|
0.33
mM
|
-N
+ 5 mM NH4
|
| MARKER
|
CLASS- IFICATION |
SUPPLEMENT
|
FINAL CONC. |
MEDIUM
|
STOCK SOL'N |
AMOUNT
ADDED to 200 ml |
| niaD
|
Rq
|
nitrite
(sodium)
|
5
mM
|
MM
|
1
M
|
1
ml
|
| "
|
Rq
|
Ammonium
|
5
mM
|
MM
|
1
M
|
1
ml
|
| "
|
Rq
|
Other
N-source
|
5
mM
|
MM
|
||
| "
|
N-u
|
Nitrate
|
10
mM
|
(=MM)
|
||
| "
|
Res
|
Chlorate
(sodium or potassium)
|
50
mM
|
-N
+ 10 mM proline, arginine or urea
|
||
| nic
|
Rq
|
nicotinamide
|
2
ug/ml
|
MM
|
0.4
mg/ml
|
100
ul
|
| "
|
Rq
|
Nicotinic
acid
|
2
ug/ml
|
MM
|
0.4
mg/ml
|
100
ul
|
| nii
|
Rq
|
ammonium
(tartrate or chloride)
|
5
mM
|
MM
|
1
M
|
1
ml
|
| "
|
Rq
|
Other
N-source
|
5
mM
|
MM
|
1
M
|
1
ml
|
| "
|
N-u
|
Nitrate
(sodium)
|
10
mM
|
MM
|
||
| "
|
N-u
|
Nitrite
(sodium)
|
10
mM
|
MM
|
||
| nir
|
Rq
|
ammonium
(tartrate or chloride)
|
5
mM
|
MM
|
1
M
|
1
ml
|
| "
|
Rq
|
Other
N-source
|
5
mM
|
MM
|
1
M
|
1
ml
|
| "
|
N-u
|
Nitrate
(sodium)
|
10
mM
|
(=MM)
|
||
| "
|
N-u
|
Nitrite
(sodium)
|
10
mM
|
MM
|
||
| "
|
Res
|
Chlorate
(sodium or potassium)
|
50
mM
|
-N
+ 10 mM proline, arginine or urea
|
||
| oli
|
Res
|
oligomycin
|
3
ug/ml
|
CM
|
||
| orn
|
Rq
|
ornithine
|
4
mM
|
MM
|
0.2
M
|
4
ml
|
| "
|
Rq
|
arginine
|
4
mM
|
MM
|
0.2
M
|
4
ml
|
| paba
|
Rq
|
p-aminobenzoic
acid
|
5
uM
|
MM
|
10
mM
|
100
ul
|
| panto
|
Rq*
|
pantothenate
|
0.1
mg/ml
|
MM
|
0.2g/ml
|
100 ul
|
| pdh
|
Rq
|
acetate
(neutral)
|
150
mM
|
-C
|
6
M
|
5
ml
|
| phen
|
Rq*
|
phenylalanine
|
4
mM
|
MM
|
0.2
M
|
4
ml
|
| pro
|
Rq
|
proline
|
4
mM
|
MM
|
0.2
M
|
4
ml
|
| "
|
Rq
|
ornithine
|
4
mM
|
MM
|
0.2
M
|
4
ml
|
| "
|
Rq
|
arginine
|
4
mM
|
MM
|
0.2
M
|
4
ml
|
| pu
|
Rq*
|
putrescine
|
60
uM
|
MM
|
12
mM
|
1
ml
|
| pyr
|
Rq*
|
uracil
|
50
ug/ml
|
MM
|
||
| pyro
|
Rq
|
pyridoxine
|
0.05
ug/ml
|
MM
|
100
ug/ml
|
100
ul
|
| ribo
|
Rq
|
riboflavin
|
0.1
ug/ml
|
MM
|
200
ug/ml
|
100
ul
|
| s
|
Rq
|
sulphite
|
2
mM
|
MM
|
0.4
M
|
1
ml
|
| "
|
Rq
|
thiosulphate
|
2
mM
|
MM
|
0.4
M
|
1
ml
|
| "
|
Rq
|
methionine
|
2
mM
|
MM
|
0.4
M
|
1
ml
|
| sB,C
|
Res
|
selenate
|
0.5
mM
|
MM
+ 0.1 mM methionine
|
1
M
|
1
ml
|
| sD,E
|
Sens
|
selenate
|
0.5
mM
|
MM
+ 0.1 mM methionine
|
1
M
|
1
ml
|
| sb
|
C-u
|
sorbitol
|
1%
|
-C
|
||
| sor
|
Res
|
sorbose
|
1.5
mg/ml
|
-CN
+ 1% glycerol + 5 mM urea |
||
| spsA
|
Sens
|
spermidine
|
10mM
|
-CN
+ 1% glycerol + 5 mM urea |
||
| sul
|
Res
|
sulphanilamide
|
1
mg/ml
|
MM
|
||
| thi
|
Rq
|
thiamine
(=aneurin)
|
0.1
ug/ml
|
MM
|
0.2
mg/ul
|
100
ul
|
| trp
|
Rq*
|
tryptophan
|
4
mM
|
MM
|
40
mM (filter sterilized)
|
20
ml
|
| ua
|
N-u
|
uric
acid
|
0.6
mM
|
-N
|
||
| "
|
N-u
|
hypoxanthine
|
100
ug/ml
|
-N
|
||
| ure
|
N-u
|
urea
|
5
mM
|
-N
|
||
| ureA
|
Res
|
thiourea
|
5
mM
|
MM
|
Haploidization of diploids
Benlate: final concentration 2ug/ml. Stock solution 200ug/ml in ethanol. Use 2ml/200ml medium
p-fluorophenylalanine: final concentration 2.5ug/ml. Stock solution 100ug/ml. Use 2.5ml/200ml medium
Reduction of background growth in nitrogen utilization tests, transformant selection etc.
Caesium chloride: final concentration 12.5mM
Conidial suspension
Tween 80: 0.01%. Stock solution 0.1%. (May inhibit growth if excess in medium)
Diethyl ether: 1% in sterile distilled water (Removes germination inhibitor, use for high density inocula)