Optimized primers and other critical
conditions for efficient fusion PCR to generate knockout vectors in filamentous
fungi: addendum.
Nicholas Harrison1, Brad Cavinder
1,2, Jeffrey P.
Townsend3, and
Frances Trail1, 4
1 Department of Plant Biology, Michigan State
University, East Lansing, MI 48824. 2Current address: Department of
Plant Pathology, University of California, Riverside. Email: brad.cavinder@ucr.edu. 3Department
of Ecology and Evolutionary Biology, Yale University, New Haven CT. Email: Jeffrey.Townsend@Yale.edu. 4Corresponding
author: trail@msu.edu
Fungal Genetics Reports 60:1-
(pdf)
The authors communicated this omission post publication:
We inadvertently left out the 3HS sequence with which we had such great success. The 5HS sequence, as seen in Figure 2D, is 5'-AGTCGACGACAACTACCATCGATCTGACG. The 3HS sequence which was missing from the original paper is 5'-ACACTGGTGACGGCTAACCAGAACTGTCA.