Genes from Aspergillus nidulans

Gene: flbA (gen)

Plasmid name: pBN29 Size in kb: 10
Resistance: amp
Depositor: Keller
Reference: Mol Microbiol 14:323-
Vector: pBS
Notes:

Gene: flbA (cDNA)

Plasmid name: pBN30 Size in kb: 5.5
Resistance: amp
Depositor: Keller
Reference: Mol Microbiol 14:323-
Vector: pBS
Notes:

Gene: fadA^d+

Plasmid name: pSM8P2 Size in kb: 7.5
Resistance: amp
Depositor: Keller
Reference: Embo J. 15:5184-5190
Vector: pSM3
Notes: pSM3 has pyroA

Gene: pyr4/AM A1

Plasmid name: pRG3- AMA1-NotI Size in kb: 10
Resistance: amp
Depositor: GSM
Reference: Genetics 155:647-656
Vector: puc19
Notes: for genomic library construction

Gene: niaD/AM A1

Plasmid name: pRG3- AMA1-niaD Size in kb: 11
Resistance: amp
Depositor: GSM
Reference: FGB 2001
Vector: puc19
Notes: for genomic library construction

Gene: niiA/AMA1

Plasmid name: pRG3- AMA1-niiA Size in kb: 11
Resistance: amp
Depositor: GSM
Reference: FGB 2001
Vector: puc19
Notes: for genomic library construction

Gene: lysB

Plasmid name: pLYSB3 Size in kb: 6.3
Resistance: Amp
Depositor: GSM
Reference:
Vector:
Notes:

Gene: argB

Plasmid name: pARGB2 Size in kb: 5.5
Resistance: Amp
Depositor: GSM
Reference:
Vector:
Notes:

Gene: Aras

Plasmid name:pAST33
Size in kb:
Resistance: amp
Depositor:Tapan Som
Reference: Mol Cell Biol 14:5333-8349
Vector: pST82
Notes: from W4B12

Gene: Aras

Plasmid name:pAST40
Size in kb: 4.2 kb
Resistance: amp
Depositor: T. Som
Reference: Mol Cell Biol 14:5333-8349
Vector: pAST30
Notes: cDNA of Aras cloned into pAST30 as NcoI-HindIII fragment
Click here for a restriction map of pAST40

Gene: actin

Plasmid name: pSF5
Size in kb: 7.8
Resistance: amp
Depositor: S. Fidel
Reference: Fidel et al 1988 Gene 70:283-293
Vector: pUC19
Notes: 5.1 HindIII insert in pUC19
Click here to see a restriction map of the pSF5 insert

Gene: acuE (malate synthase)

Plasmid name: pRAS12
Size in kb: 12.5
Resistance: amp
Depositor: M. Hynes
Reference: Sandeman and Hynes 1989 MGG 218:87-92
Vector: pBR322
Notes: 8 kb Xho fragment into Sal cut pBR322
Click here to see a restriction map of the pRAS12 insert

Gene: alcA

Plasmid name: pAST30
Size in kb: 3.1
Resistance: amp
Depositor: Tapan Som
Reference: Mol Cell Biol 14:5333-5348
Click here for a restriction map of pAST30

Gene: amdR (acetamidase regulation)

Plasmid name: pALX-1
Size in kb: 14.3
Resistance: amp
Depositor: M. Hynes
Reference: Andrianopoulos and Hynes 1988. MCB 8:3532-3541
Vector: pBR322
Notes: 10 kb BglII fragment into BamHI cut pBR322

Gene: amdS (acetamidase)

Plasmid name: p3SR2
Size in kb: 9.3
Resistance: amp
Depositor: M. Hynes
Reference: Wernars et al 1985. Curr Genet 9:361-368
Vector: pBR322
Notes: 5 kb EcoRI/Sal fragment into pBR322

Gene: areA (ammonium regulation)

Plasmid name: pAR4-322-1
Size in kb: 15.7
Resistance: amp
Depositor: M.J. Hynes
Reference: M.A. Davis and M.J. Hynes, unpublished
Vector: pBR322
Notes: 14 kb EcoRI frag in the EcoRI site of pBR322

Gene: argB (ornithine transcarbamylase)

Plasmid name: pILJ16
Size in kb: 5.5
Resistance: amp
Depositor: I.L. Johnstone
Reference: Johnstone et al 1985. EMBO J 4:1307
Vector: pUC8
Notes: argB+ on 2.7 kb SalI fragment
Click here to see a restriction map of pILJ16

Gene: argB (ornithine transcarbamylase)

Plasmid name: pDC1
Size in kb: 4.5
Resistance: amp
Depositor: R. Aramayo
Reference: Aramayo et al 1989. Genetics 122:65-71
Vector: pIC20R
Notes: 1.8 kb SphI-BamHI framgent into pIC20R
Click here to see a restriction map of pDC1

Gene: argB (ornithine transcarbamylase)

Plasmid name: pMS12
Size in kb: 4.7
Resistance: amp
Depositor: M. Stringer
Reference:
Vector: pBS(+)
Notes: argB on 1.7 kb insert
Click here to see a restriction map of pMS12

Gene: benA (beta-tubulin)

Plasmid name: pB-5
Size in kb: 8.1
Resistance: amp
Depositor: G. May
Reference: May et al 1987. Gene 55:231-243
Vector: pUC19
Notes: obtained from Greg May 10-5-92 5.4 kb PstI frg of beta5 in pUC19

Gene: bimC

Plasmid name: pAEBamI
Size in kb: 6.7
Resistance: amp
Depositor: R. Morris
Reference: Enos and Morris 1990 Cell 60:1019-1027
Vector: pUC18
Notes: 4 kb HindIII fragment containing bimC in pUC18

Gene: bimG (protein phosphatase)

Plasmid name: pBG4
Size in kb: 7.6?
Resistance: amp
Depositor: R. Morris
Reference: Doonan and Morris 1989 Cell 57:987-996
Vector: pGM3
Notes: 2.5 kb EcoRI fragment containing bimG in pGM3 (pBR322 with pyr-4)

Gene: calmodulin-dependent protein Kinase

Plasmid name: p4b11
Size in kb: 4.7
Resistance: amp
Depositor: D. Bartelt
Reference: Gene 113:75-82
Vector: pBluescript SK+

Gene: facA (acetyl CoA synthetase)

Plasmid name: pRAS7
Size in kb: 10.3
Resistance: amp
Depositor: M. Hynes
Reference: Sandeman and Hynes 1989 MGG 218:87-92
Vector: pBR322
Notes: 6 kb Xho fragment into Sal cut pBR322

Gene: gdhA (NADP glutamate dehydrogenase)

Plasmid name: pSJG1
Size in kb: 5.4
Resistance: amp
Depositor: S.J. Gurr
Reference: Gurr et al 1986. Curr Genet 10:761-766
Vector: pAT153
Click here to see a restriction map of pSJG1

Gene: lamA (lactamase)

Plasmid name: plam7
Size in kb: 14.8
Resistance: amp
Depositor: M. Hynes
Reference: Katz and Hynes 1989. Genetics 122:331-339
Vector: pHY201
Notes: rescued from transformed E. coli, so could be modified

Gene: mipA (gamma tubulin)

Plasmid name: pLO6
Size in kb: 5.5
Resistance: amp
Depositor: B. Oakley
Reference: Oakley and Oakley 1989 Nature 338:662
Vector: pUC19
Notes: 2.8 HindIII-SacI genomic fragment containing gamma-tubulin

Gene: mipA (gamma tubulin)

Plasmid name: pTH2
Size in kb: 4.5
Resistance: amp
Depositor: B. Oakley
Reference: Oakley and Oakley 1989 Nature 338:662
Vector: pBS(KS+)
Notes: full length DNA (1.5 kb EcoRI frag) ligated in pBS KS+
Click here to see a restriction map of pTH2

Gene: nimA (protein kinase)

Plasmid name: pSAO1
Size in kb: 8.1
Resistance: amp
Depositor: R. Morris
Reference: Osmani et al. 1987 J. Cell Biol. 104:1495-1504
Vector: pUC18
Notes: 5.4 kb KpnI fragment containing nimA in pUC18

Gene: nudC

Plasmid name: pnudC/6.4
Size in kb: 9.1
Resistance: amp
Depositor: R. Morris
Reference: Osmani et al. 1990 J. Cell Biol. 111:543-551
Vector: pUC18
Notes: 6.4 kb EcoRI fragment containing nudC in pUC18

Gene: pyrG (orotidine decarboxylase)

Plasmid name: ppyrG
Size in kb: 4.8
Resistance: amp
Depositor: G. May
Reference:
Vector: pUC19
Notes: EcoRI, BamH1, HindIII linearize

Gene: pyrG (orotidine decarboxylase)

Plasmid name: pJR15
Size in kb: 6.5
Resistance: amp
Depositor: B. Oakley
Reference: Oakley et al 1987. Gene 61:385-399
Vector: pBR329
Notes: Their #BROB43, in DH1

Gene: riboB

Plasmid name: pLO1
Size in kb: 12.0
Resistance: cap
Depositor: Oakley
Reference: Oakley et al 1987. Gene 53:293-298
Vector: pBR329
Notes: Their #BROB46, in DH1

Gene: riboB

Plasmid name: pPL1
Size in kb: 6.8
Resistance: amp
Depositor: Oakley
Reference: Oakley et al 1987. Gene 53:293-298
Vector: pUC19
Notes: Their #BROB63, in JM109

Gene: rodA

Plasmid name: pTA51
Size in kb: 6.7
Resistance: amp
Depositor: Mary Stringer
Reference: Stringer et al 1991 Genes Dev. 5:1161-1171
Vector: pBS KS-
Notes:

Gene: rps16

Plasmid name: prps16
Size in kb: 5.3
Resistance: amp
Depositor: R. Bradshaw
Reference: Bradshaw and Pillar 1991 Gene 108:157-162
Vector: pBlueScr

Gene: trpC (anthranilate synthetase, IGP synthetase)

Plasmid name: pHY201
Size in kb: 8.2
Resistance: amp
Depositor: W. Timberlake
Reference: Mullaney et al 1985. MGG 199:37-45
Click here to see a restriction map of pHY201

Gene: tubA (alpha-tubulin)

Plasmid name: pDP485
Size in kb: 7.6
Resistance: amp
Depositor: R. Morris
Reference: Doshi et al. 1991 MGG 225:129-141
Vector: pUC19
Notes: 4.85 kb SacI fragment containing tubA in pUC19

Gene: tubB (alpha-tubulin)

Plasmid name: pDP6031
Size in kb: 8.2
Resistance: amp
Depositor: R. Morris
Reference: Doshi et al. 1991 MGG 225:129-141
Vector: pUC19
Notes: 5.5 kb HindIII fragment containing tubB in pUC19

Gene: tubC (beta-tubulin)

Plasmid name: pB-14-2
Size in kb: 5.9
Resistance: amp
Depositor: G. May
Reference: May et al. 1987. Gene 55:231-243
Vector: pUC19
Notes: obtained from Greg May 10-5-92 3.2 kb XhoI frag in pUC19

Gene: wA

Plasmid name: pNK3
Size in kb: 16.5
Resistance: cap
Depositor: M. Mayorga
Reference: Mayorga and Timberlake 1992 Genetics 126:73-79
Vector: pUC13cam
Click here for a restriction map of pNK3

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Last modified 11/98 KMC