Reporter Constructs at the FGSC

Reporter gene plasmids

(GFP, RFP, BFP, YFP, Luciferase)

Gene: GFP: Plasmid name: gGFP Size in kb: 9.24
Resistance: amp
Depositor: AS
Reference: Mycol Res 102:491-496
Notes: designed for Cochliobolus

Gene: GFP: Plasmid name: pMF272 Size in kb: 8.4
Resistance: amp
Depositor: MHF
Reference: Freitag et al. Fungal Genet Biol. 2004 Oct;41(10):897-910
Vector: pBM61
Gene: hH1-GFP: Plasmid name: pMF280 Size in kb: 9.5
Resistance: amp
Depositor: MHF
Reference: Freitag et al. Fungal Genet Biol. 2004 Oct;41(10):897-910
Vector: pBM61
Gene: Bml-GFP: Plasmid name: pMF309 Size in kb: 10.4
Resistance: amp
Depositor: MHF
Reference: Freitag et al. Fungal Genet Biol. 2004 Oct;41(10):897-910
Vector: pBM61

Aspergillus
Gene: GFP: Plasmid name: pFNO3 Size in kb: 6.5
Resistance: Kan
Depositor: SO
Reference: Euk Cell 3:1359-1362
Vector:
Notes: GA5-GFP + A. fumigatus pyrG
http://www.fgsc.net/plasmid/pfno3_web.doc
Gene: GFP: Plasmid name: pRF280 Size in kb:
Resistance: amp
Depositor: RF
Reference: Toews et al, 2004
Vector:
Notes: gpd::GFP::stuA; argB, labels nuclei
Gene: GFP: Plasmid name: pRF281 Size in kb:
Resistance: amp
Depositor: RF
Reference: Toews et al, 2004
Vector:
Notes: gpd::GFP::stuA; pyr4, labels nuclei
Gene: GFP: Plasmid name: pRS54 Size in kb:
Resistance: amp
Depositor: RF
Reference: R. Fischer's page
Vector:
Notes: gpd::cit::GFP, labels mitochondria
Gene: sGFP: Plasmid name: pMT-sGFP Size in kb:
Resistance: amp
Depositor: RF
Reference: Toews et al, 2004
Vector:
Notes: alcA::ccdB::sGFP
Gene: sGFP: Plasmid name: pDV2 Size in kb:
Resistance: amp
Depositor: RF
Reference: R. Fischer's page
Vector:
Notes: gpd::ccdB::sGFP

RFP

Neurospora

Gene: RFP: Name: pMF331
Resistance: amp
Depositor: MHF
Reference: Fungal Genet. Newsl. 52:14-17
Notes: Genbank DQ250997
Gene: RFP: Name: pMF332
Resistance: amp
Depositor: MHF
Reference: Fungal Genet. Newsl. 52:14-17
Notes: Genbank DQ250998
Gene: RFP: Name: pMF334
Resistance: amp
Depositor: MHF
Reference: Fungal Genet. Newsl. 52:14-17
Notes: Genbank DQ250999

Aspergillus

Gene: DsRedT4: Plasmid name: pJH19 Size in kb:
Resistance: amp
Depositor: RF
Reference: Toews et al, 2004
Notes: gpd::DsRedT4::stuA; argB, labels nuclei
Gene: RFP: Plasmid name: pXDRFP4 Size in kb: 6.2
Resistance: Kan
Depositor: SO
Reference: Euk Cell 3:1359-1362
Notes: GA5-mRFP + A. fumigatus pyrG
http://www.fgsc.net/plasmid/pXDRFP4_web.doc
Gene: DsRedT4: Plasmid name: pSK700 Size in kb:
Resistance: amp
Depositor: RF
Reference: Toews et al, 2004
Notes: gpd::cit::DsRedT4, labels mitochondria
Gene: mRFP1: Plasmid name: pPND1 Size in kb:
Resistance: amp
Depositor: RF
Reference: R. Fischer's page
Notes: alcA::mRFP1::KipB, labels microtubules
Gene: alcA::ccdB: Plasmid name: pMT-OvE Size in kb:
Resistance: amp
Depositor: RF
Reference: Toews et al, 2004
Notes: alcA::ccdB
Gene: mRFP1: Plasmid name: pMT-mRFP1 Size in kb:
Resistance: amp
Depositor: RF
Reference: Toews et al, 2004
Notes: alcA::ccdB::mRFP1
Gene: mRFP1: Plasmid name: pSK800 Size in kb:
Resistance: amp
Depositor: RF
Reference: Toews et al, 2004
Notes: gpd::cit::mRFP1, labels mitochondria

BFP

Gene: BFP: Plasmid name: pMT-BFP Size in kb:
Resistance: amp
Depositor: RF
Reference: Toews et al, 2004
Vector:
Notes: alcA::ccdB::BFP

YFP

Gene: YFP: Plasmid name: pYFP Size in kb: 8.4
Resistance: amp
Depositor: P. Shiu
Reference: Genetics 178:593-596
Vector: pMF272
Notes: genbank #EF661030
Gene: YFPN: Plasmid name: pYFPN Size in kb: 8.2
Resistance: amp
Depositor: P. Shiu
Reference: Genetics 178:593-596
Vector: pMF272
Notes: genbank #EF661031
Gene: YFPC: Plasmid name: pYFPC Size in kb: 8.0
Resistance: amp
Depositor: P. Shiu
Reference: Genetics 178:593-596
Vector: pMF272
Notes: genbank #EF661032

Luciferase

Gene: lux: Plasmid name: pLUC6 Size in kb: 8.4
Resistance: amp
Depositor: D. Bell-Pedersen
Reference: FGB 38:327-332
Map:
Notes: The BstEII site in his-3 was mutated to allow removal of the ccg-2 promoter using BstEII at the 3' end
Gene: luc: Plasmid name: pBM61-ccg2p-o-luc-I (VG101)
Size in kb: 9.3
Resistance: amp
Depositor: J. Dunlap /A. Mehra
Reference: Euk. Cell 7:28-37; Vector: pBM61
Map: http://www.fgsc.net/plasmid/VG101.jpg
Gene: luc: Plasmid name: pBM61-frqp-o-luc-I (VG110)
Size in kb: 11.5
Resistance: amp
Depositor: J. Dunlap /A. Mehra
Reference: Euk. Cell 7:28-37
Vector: pBM61
Map: http://www.fgsc.net/plasmid/VG110.jpg

Protein Tag

Gene: 3xHA: Plasmid name: pMT-3xHA Size in kb:
Resistance: amp
Depositor: RF
Reference: Toews et al, 2004
Vector:
Notes: alcA::ccdB::3xHA

Last modified 4/08 KMC