quinate-2

locus: qa-2

locus_name: quinate-2

organism_type: B

chromosome_number: VII

chromosome_side:

link_group: VII

cultural_requirements: VII. In the qa cluster between qa-4 and met-7 (0.02%) (146). (884) Lacks catabolic dehydroquinase (148, 517, 884). Unable to use quinate or shikimate as the sole carbon source in the presence of aro-9, which results in the absence of biosynthetic dehydroquinase (885) (Fig. 21). aro-9;qa-2+ strains grow on minimal medium without supplement. qa-2 is conveniently scored as an aro auxotroph when aro-9 is present. qa-2+ cloned in pBR322 (pVK57) is expressed constitutively from its own promoter in E. coli(23, 388, 451); has been returned to Neurospora chromosomal sites by transformation (150). Allele M246 is stable (150).

enzyme_id: 81

Enzyme: catabolic dehydroquinase

anid_name: qutE

near_l: qa-4

near_r: met-7 (0.02%)

neu_name:

supplement:

image_url:

Strains

FGSC #	Reporting Genes	Species
FGSC #2718	qa-2;aro-9	Neurospora crassa
FGSC #3230	pan-2;qa-2	Neurospora crassa
FGSC #3231	pan-2;qa-2	Neurospora crassa
FGSC #3952	qa-2;aro-9;inl	Neurospora crassa
FGSC #3957	aro-9;qa-2	Neurospora crassa
FGSC #3958	aro-9;qa-2	Neurospora crassa
FGSC #4545	trp-1;qa-2;aro-9;inl	Neurospora crassa
FGSC #4546	trp-3 aro-9;qa-2	Neurospora crassa
FGSC #8340	smco-7 qa-2 aro-9 inl	Neurospora crassa

References

Authors	Title	Year
Alton, N. K. , J. A. Hautala, N. H. Giles, S. R. Kushner, and D. Vapnek.	Transcription and translation in E. coli of hybrid plasmids containing the catabolic dehydro- quinase gene from N. crassa.	1978
Case. M. E. , and N. H. Giles.	Gene order in the qa gene cluster of N. crassa.	1976
Case, M. E. , J. A. Hautala, and N. H. Giles.	Characterization of qa-2 mutants of N. crassa by genetic, enzymatic, and immunologicaltechniques.	1977
Case, M. E. , M. Schweizer, S. R. Kushner, and N. H. Giles.	Efficient transformation of N. crassa utilizing hybrid plasmid DNA.	1979
Giles, N. H. , N. K. Alton, M. E. Case, J. A. Hautala, J. W. Jacobson, S. R. Kushner, V. B. Patel, W. R. Reinert, P. Stroman, and D. Vapnek.	The organization of the qa gene cluster in Neurospora crassa and its expression in Escherichia coli.	1978
Hautala, J. A. , M. Schweizer, N. H. Giles, and S. R. Kushner.	Constitutive expression in Escherichia coli of the Neurospora crassastructural gene encoding the inducible enzyme catabolic dehydroquinase.	1979
Jacobson, J. W. , J. A. Hautala, M. E. Case, and N. H. Giles.	Effect of mutations in the qa gene cluster of Neurospora crassa on theenzyme catabolic dehydroquinase.	1975
Rines, H. W.	Genetical and biochemical studies on the inducible quinic acid catabolic pathway in Neurospora crassa.	1969
Rines, H. W. , M. E. Case, and N. H. Giles.	Mutants in the arom gene cluster of Neurospora crassa specific for biosyntheticdehydroquinase.	1969
Geever et al		1989
Giles et al		1985