enhancer-2 of am

locus: en(am)-2

locus_name: enhancer-2 of am

organism_type: B

chromosome_number: II

chromosome_side: R

link_group: IIR

cultural_requirements: IIR. Linked near pe (983).In en(am)-2;am double mutants, en(am)-2 counteracts leakiness of am on minimal medium. en(am)-2;am strains grow well on L-alanine (25 mM) or 0.5% casein hydrolysate (983) or on glutamate (5 mM) (293). The single mutant en(am)-2without am grows normally on minimal medium. Mutant en(am)-2 lacks glutamate synthase (GOGAT) (see Fig. 19). The double mutant en(am)-2;am lacks NADP-glutamate dehydrogenase and GOGAT activities (293). Frequent revertants of the double mutant en(am)-2;am on suboptimal medium are attributed to back-mutation at am (983). Formerly called en-am.

enzyme_id: 110

Enzyme: glutamate synthase

anid_name: amaA

near_l: Linked near pe

near_r:

neu_name:

supplement: L-alanine

image_url:

Strains

FGSC #	Reporting Genes	Species
FGSC #1625	en(am)-2;am;pe	Neurospora crassa

References

Authors	Title	Year
Dunn-Coleman, N. S. , E. A. Robey, A. B. Tomsett, and R. H. Garrett.	Glutamate synthase levels in Neurospora crassa mutants alteredwith respect to nitrogen metabolism.	1981
Shields, M.	A mutation which prevents adaptation of am mutants of Neurospora crassa.	1968