osmotic sensitive

locus: os

locus_name: osmotic sensitive

organism_type: B

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cultural_requirements: Unable to grow on media with elevated osmotic pressure. Scorable on solid or liquid media plus 4% NaCl (1.4 M). Most alleles can also be scored by morphology, having sticky, close-cropped aerial hyphae that tend to rupture and bleed. Morphology is influenced by humidity. Intense pigment of aggregated hyphae has suggested the name "flame," which was originally applied to some os mutants. os strains are useful for obtaining protoplasts (e.g., reference 971) and are reported to be efficient as recipients for DNA-mediated transformation in media of high osmolarity (1162). In addition to the numerous loci designated os, cut is a typical osmotic mutation. Mutant sor(T9) is osmotic sensitive and sorbose resistant and has low glucoamylase activity, but does not show the typical os morphology.

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Strains

FGSC #	Reporting Genes	Species

References

Authors	Title	Year
Selitrennikoff, C. P. , B. L. Lilley, and R. Zucker.	Formation and regeneration of protoplasts derived from a temperature-sensitiveosmotic strain of Neurospora crassa.	1981
Wootton, J. C. , M. J. Fraser, and A. J. Baron.	Efficient transformation of germinating Neurospora conidia using total nuclear DNAfragments.	1980