Gene: nitrate nonutilizer
nitrate nonutilizer
locus: nit
locus_name: nitrate nonutilizer
organism_type: B
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cultural_requirements: Conveniently scored on synthetic crossing medium (1134), in which nitrate is the sole nitrogen source. Also scorable on slants by pH change when grown with ammonium nitrate as the nitrogen source and bromcresol purple (4 mg/ml) as an indicator (791). In most crosses, a nit mutant can be used as the fertilizing parent; in nit x nit or other crosses where it is required as the female parent, crossing medium can be altered by substituting ammonium nitrate for potassium nitrate (155). Nitrite is toxic at low pH; test media containing nitrite should be neutralized, and the nitrite should preferably be filter-sterilized (G.S. Sorger, personal communication). For a summary of nutritional requirements, based on the data of various authors, see reference 1080. nit-1, nit-7, nit-8, and nit-9 involve a molybdenum-containing cofactor common to nitrate reductase and xanthine dehydrogenase (591, 1080, 1081) (Fig. 19 and 24). For a review of nitrate assimilation, see reference 385. For regulation, see reference 643 (review), references 292, 835, 837, and 1081, and entries for nitloci, gln-1, and nmr. Nitrate reduction pathway showing sites of gene action. am only blocks the NADP-specific glutamate dehydrogenase, not the NAD-specific enzyme. (185, 293, 336, 591, 912, 999, 1081)
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Strains
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References
