Gene: quinate utilization VII
quinate utilization
locus: qa
locus_name: quinate utilization
organism_type: B
chromosome_number: VII
chromosome_side:
link_group: VII
cultural_requirements: Gene cluster located in linkage group VII (177, 884), in the order: qa-1(0.02%) qa-3 qa-4 qa-2 (0.02%) met-7 (146). No recombination with the centromere has been observed in several hundred asci; qa has been tentatively shown right of the centromere, on the basis of close linkage to met-7 (146; M.E. Case, personal communication). The gene cluster functions in the quinate catabolic pathway. Mutants selected by inability to use quinate as their sole source of carbon (178, 179, 885). The enzymes of the quinate pathway are induced when quinate is present in the medium; but see also reference 423 and references therein. Scored by the ability to use quinic acid (0.3%) as the sole carbon source (146). qa-1 encodes a regulatory protein; the other qa genes encode inducible enzymes (179). The first and second steps of the quinate pathway are paralleled by comparable reactions in the aromatic biosynthetic pathway (Fig. 11). Thus, the enzymes encoded by aro-9 and qa-2can substitute for each other, and under appropriate conditions the qa-3enzyme can substitute for the aro-1 enzyme (147). Separate transcripts are made by components of the qa cluster, rather than a single polycistronic messenger RNA (387, 781). For a diagram of the catabolic pathway and its relation to the biosynthetic pathway, see references 146, 387, and 423 and Fig. 21. The entire cluster has been cloned in E. coliand returned to Neurospora by transformation (941). The cluster consists of adjacent coding sequences totaling about 18 kilobases. A transcriptional map has been constructed, and two new genes of unknown function, qa-x and qa-y, have been identified from messenger RNAs (781). For regulation, see references 179, 423, 781, and 873.
enzyme_id:
anid_name:
near_l: in the order: qa-1(0.02%) qa-3 qa-4 qa-2 (0.02%), met-7
near_r:
neu_name:
supplement:
image_url:
Strains
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References
