Gene: slime
slime
locus: slime
locus_name: slime
organism_type: B
chromosome_number:
chromosome_side:
link_group:
cultural_requirements: A multiple-mutant strain lacking cell wall and growing as protoplasts or plasmodium. The original strain contained at least two mutations (fz, fuzzy; sg, slow germination) in addition to the markers already present, arg-1, cr-1, aur, and os-1. Of these, fz, sg, and os-1 are required for a slime-like phenotype (321). Can be recovered with inserted markers in f1 of crosses if filtration enrichment is used (746). Cell wall-like material may be produced in small quantities in newly resolved stocks. Loses ability to form heterokaryons or to function as the fertilizing parent after continuous growth (973). Slime protoplasts can be induced to fuse (743). Stocks maintained in same-mating type (321) or mixed-mating type (746) heterokaryons or frozen at -70°C in situ on agar medium (968) or in dimethyl sulfoxide (221) or growth medium (961). Recovered from heterokaryons by filtration; see reference 746. Plasma membrane can be isolated and stored in large quantity (1037). Used for fatty acid analysis of plasma membrane (365), for study of vacuoles (922, 638), and for gentle extraction of enzymes (372). Used to show that polyadenylic acid polymerase and nuclease activities are largely located in the nucleus (962). For general methodology, see references 746 and 1161. For an alternative source of cell wall-free Neurospora, see references 970 and 971.
enzyme_id:
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near_r:
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Strains
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References
