Gene: tetrazolium IL
tetrazolium
locus: tet
locus_name: tetrazolium
organism_type: B
chromosome_number: I
chromosome_side: L
link_group: IL
cultural_requirements: IL. Right of mating type (7 to 8%). Linked to acr-3 (2%) and ad-3B (1%) (395). Tetrazolium dye reduction. Detected as a difference between A and a laboratory strains (74-OR23-1A colonies fail to reduce dye and thus remain white; 74-OR8-1a colonies reduce dye to red) (395). Called Tet-R and Tet-W ("red," "white"). See reference 395 for tests on other wild types. cya, cyb, and cyt mutants all fail to reduce 2,3,5-triphenyl-tetrazolium chloride, and this is used as a test in the initial identification of such mutants. cya-1, cyt-3, and cyt-4 are all located near the mating type locus (87); their relationship to tet is not known. Probably (but not certainly) the same gene is responsible for the mating-type-linked difference in resistance to 2,3,5-triphenyl-tetrazolium chloride. 2,3,5-triphenyl-tetrazolium chloride-resistant strains include 74-OR23-1A, Em A FGSC no. 691, Em A 5256, Lindegren 1A, Lindegren 25a, and all RL wild types tested. 2,3,5-Triphenyl-tetrazolium-sensitive strains include 74-OR8-1a, Em a FGSC no. 692, and Em a 5297 (1116, 1117). Oak Ridge a wild-type strain ORSa, derived by backcrossing to 74-OR23-1A, is resistant (731). The gene for 2,3,5-triphenyltetrazolium chloride resistance maps left of mei-3, probably between mt and arg-1 (D.R. Galeazzi, personal communication).
enzyme_id:
anid_name:
near_l: mating type (7 to 8%)
near_r: Linked to acr-3 (2%) and ad-3B (1%)
neu_name:
supplement:
image_url:
Strains
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References
