Gene: urease
urease
locus: ure
locus_name: urease
organism_type: B
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cultural_requirements: Strains carrying mutations at four distinct loci, ure-1, -2, -3, -4, lack all detectable urease activity (Fig. 10 and 24). Urease from other organisms comprises numerous distinct subunits; possibly all four Neurospora loci are structural genes (78). uremutation D2, which is tightly linked to ure-1, fails to complement mutations at any of the four loci, suggesting a regulatory role (452). Strains carrying mutations of another type possess partial activity but are readily scorable by poor growth on urea as the nitrogen source. These probably represent additional loci in V (A7, S3), IV (E3, E7), or elsewhere (C5, K3, R2) (452; H.B. Howe, Jr., personal communication). They are not given separate entries here. ure mutants have been isolated by methods based on the inability to generate ammonia from urea, using pH indicators (78, 452, 569). The following methods for scoring isolates are probably generally applicable, but all have not been tested on mutations at all loci. Method 1: Little growth on filter-sterilized urea as the sole nitrogen source (452) (not good if amino acids must be added). Method 2: Five- to twenty-minute scoring test, touching bits of filter paper dipped in urea-bromthymol blue buffer to conidia or aerial growth (570). Method 3: Color change when grown on slants of synthetic crossing medium (1134) containing phenol red (90 ug/ml) and urea (3 mg/ml) as the sole nitrogen source, added before autoclaving; scored after 4 to 5 days at 34°C (PB; modified from the method of J.A. Kinsey).
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Strains
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References
