locus: nkuB::A. fumigatus riboB

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organism_type: A

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cultural_requirements: Riboflavin requirement. The nkuA gene was deleted in KJ12 by replacing it with argB. This was crossed to A770. TN02A7 is a segregant of this cross. NkuB was deleted in TN02A7 by replacing it with the A. fumigatus riboB gene. NkuA and nkuB form a heterodimer important for non-homologous end joining of DNA. Deletion of nkuA greatly reduces the frequency of insertion of transforming DNA by non-homologous integration and, thus, greatly facilitates gene targeting by homologous recombination. Deletion of nkuB (by replacement with the A. fumigatus riboB gene) in an nkuA deletion background does not cause any improvement in gene targeting relative to an nkuA deletion. NkuA/nkuB double deletion strains show no obvious difference in growth or sensitivity to mutagens when compared to nkuA single deletion ro wild-type strains.

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Strains

FGSC #	Reporting Genes	Species
FGSC A1148	pyrG89; nkuB::A. fumigatus riboB pyroA4 nkuA::argB; riboB2	Aspergillus nidulans

References

Authors	Title	Year