Gene: cysteine-3 IIL

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cysteine-3

locus: cys-3

locus_name: cysteine-3

organism_type: B

chromosome_number: II

chromosome_side: L

link_group: IIL

cultural_requirements: IIL. Right of pi (4%). Left of T(AR18) and pyr-4 (18 to 21%) (721, 808, 816).Uses sulfite, cysteine, or methionine; little or no response to thiosulfate (640, 721). Regulator of genes of sulfur uptake and metabolism (e.g., sulfate permease, aryl sulfatase, choline sulfatase) (284, 640, 667). Grows well on methionine. Resistant to chromate (640). Used extensively for studying regulation; for a review, see reference 642. cys-3 ascospores darken slowly or not at all, even when a cys-3 strain is the fertilizing parent and when a strain carrying heat-sensitive allele NM27t is crossed at 25°C, the permissive temperature for growth. Adding methionine to crossing medium promotes darkening but fails to give good recovery of cys-3 progeny. Recovery of a few percent cys-3 progeny is possible in well-aged crosses. (721, PB). cys-3 can be used effectively as an autonomous ascospore color mutant for demonstrating segregation patterns in asci (811; see reference 858 for photograph; see Ascospore color mutants).

enzyme_id: 202

Enzyme: none

anid_name:

near_l: pi (4%)

near_r: T(AR18) and pyr-4 (18 to 21%)

neu_name:

supplement: sulfite or cysteine or methionine

image_url:

Strains

FGSC # Reporting Genes Species
FGSC #11Has been replaced by strain number: 1090Neurospora crassa
FGSC #1089Has been replaced by strain number: 4028Neurospora crassa
FGSC #1090Has been replaced by strain number: 4029Neurospora crassa
FGSC #1272cys-3Neurospora crassa
FGSC #1289Not available; cys-3 flNeurospora crassa
FGSC #1290cys-3 arg-5Neurospora crassa
FGSC #2292cys-3Neurospora crassa
FGSC #4028cys-3Neurospora crassa
FGSC #4029cys-3Neurospora crassa
FGSC #4149cys-3 arg-5Neurospora crassa
FGSC #4689cys-3 easNeurospora crassa
FGSC #4690cys-3 easNeurospora crassa

References

Authors Title Year
Dietrich, P. S. , and R. L. Metzenberg.Metabolic suppressors of a regulatory mutant in Neurospora.1973
Marzluf, G. A.Genetic and metabolic controls for sulfate metabolism in Neurospora crassa:isolation and study of chromate-resistantand sulfate transport-negative mutants.1970
Metzenberg, R. L. , and S. K. Ahlgren.Structural and regulatory control of aryl sulfatase in Neurospora:the use of interspecificdifferences in structural genes.1971
Murray, N. E.Cysteine mutant strains of Neurospora.1965
Perkins, D. D. , and E. G. Barry.The cytogenetics of Neurospora.1977
Perkins, D. D. , and M. Bjorkman.Additional special purpose stocks.1979
Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett.New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage.1969
Raju, N. B.Meiosis and ascospore genesis in Neurospora.1980

Neurospora Crassa Wikipedia

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