leucine

locus: leu

locus_name: leucine

organism_type: B

chromosome_number:

chromosome_side:

link_group:

cultural_requirements: For biosynthetic pathway, see Fig. 15. Leucine mutants have been used extensively for studies of regulation (see references 427 and 833). Leucine mutants acquire suppressors when grown on Difco agar-sorbose medium. The suppressors are leaky auxotrophs blocked at various steps in sulfur metabolism; apparently these blocks allow more efficient use of the traces of leucine in the agar (425; S. R. Gross, personal communication). Most aliphatic and aromatic amino acids can inhibit growth of leucine mutants at appropriate concentrations, probably because of competition for a common uptake system (S. R. Gross, personal communication). For regulation, see individual loci; reviewed in references 427 and 642.

enzyme_id:

anid_name:

near_l:

near_r:

neu_name:

supplement: leucine

image_url:

Strains

FGSC #	Reporting Genes	Species
FGSC A965	cspA1; olvA1; bioB2; leu A1; niaD2	Aspergillus niger
FGSC #2512	kyn-1;leu	Neurospora crassa
FGSC A1010	A flavus White leu aflR pyrG	Aspergillus flavus

References

Authors	Title	Year
Gross, S. R.	A selection method for mutants requiring sulfur-containing compounds for growth.	1962
Gross, S. R.	Genetic regulatory mechanisms in the fungi.	1969
Polacco, J. C. , and S. R. Gross.	The product of the leu-3 cistron as a regulatory element for the production of the leucine biosyntheticenzymes of Neurospora.	1973