nitrate nonutilizer-2

locus: nit-2

locus_name: nitrate nonutilizer-2

organism_type: B

chromosome_number: I

chromosome_side: L

link_group: IL

cultural_requirements: IL. Right of the T(39311) left breakpoint and of un-5(2%). Left of In(OY323) and leu-3 (12 to 18%) (57, 808, 816, PB). (335, 1135) Cannot use nitrate, nitrite, purines, or most amino acids as a nitrogen source but will grow on ammonia, glutamine, or glutamate. nit-2+ is a major nitrogen control gene and mediates nitrogen catabolite repression. The nit-2 mutant is missing (or has severely reduced levels of) nitrate reductase, nitrite reductase, uricase, xanthine dehydrogenase, allantoinase, allantoicase, L-amino acid oxidase, general amino acid permease, extracellular protease, and an intracellular neutral phenylmethylsulfonyl fluoride-sensitive protease (227, 324, 441, 872, 1001, and references therein). Also affects levels of glutamate dehydrogenases (226) and uptake of uracil and uridine (128). Prevents leaky growth of the mutant am on minimal medium (155). The product of the nit-2gene has been tentatively identified as a nuclear DNA-binding protein, whose affinity for DNA is reduced in the presence of glutamine (433). Allele K31 (called pink) originated in N. sitophila and was introgressed into N. crassa (335); protein product of K31 may show altered mobility (433). Recombination within the nit-2 locus is subject to regulation by rec-1 (157). Heterozygosity for closely linked ss reduces recombination within nit-2 (161). Called amr: ammonium regulation in reference 872.

enzyme_id: 170

Enzyme: regulatory

anid_name: areAtamA

near_l: T(39311) left breakpoint and un-5(2%)

near_r: In(OY323) and leu-3 (12 to 18%)

neu_name:

supplement:

image_url:

Strains

FGSC #	Reporting Genes	Species
FGSC #33	nit-2	Neurospora sitophila
FGSC #982	nit-2	Neurospora crassa
FGSC #983	nit-2	Neurospora crassa
FGSC #2698	nit-2	Neurospora crassa
FGSC #4288	T(I->V)OY321,nit-2 leu-3	Neurospora crassa
FGSC #4289	T(I->V)OY321,nit-2 leu-3	Neurospora crassa
FGSC #5381	T(VL->IL)QNS-6(OY321),nit-2 leu-3;caf-1 at	Neurospora crassa
FGSC #5960	F. verticillioides nit2	Fusarium verticillioides
FGSC #5965	F. verticillioides nit2	Fusarium verticillioides
FGSC #6024	nit-2 leu-3	Neurospora crassa
FGSC #6029	ssu-1;nit-2;inl	Neurospora crassa
FGSC #6036	nit-2;inl	Neurospora crassa
FGSC #7224	ro-10 nit-2 leu-3	Neurospora crassa
FGSC #7225	ro-10 nit-2 leu-3	Neurospora crassa
FGSC #7226	nit-2 leu-3	Neurospora crassa
FGSC #7240	un-5 nit-2	Neurospora crassa
FGSC #7868	(fr nit-2 A + a[m1] ad-3B cyh-1)	Neurospora crassa
FGSC #9546	nit-2	Neurospora crassa

References

Authors	Title	Year
Barry, E. G. , and J. F. Leslie.	An interstitial pericentric inversion in Neurospora.	1982
Buxton, F. P. , and A. Radford.	Nitrogen metabolic repression of fluoropyrimidine resistance and pyrimidine uptake in N. crassa.	1982
Catcheside, D. E. A.	Control of recombination within the nitrate-2 locus of N. crassa: an unlinked dominant gene which reduces prototroph yields.	1970
Catcheside. D. E. A.	A second locus subject to recombination control by the rec-1+ gene in N. crassa.	1974
Catcheside, D. E. A.	Genes in Neurospora that suppress recombination when they are heterozygous.	1981
Dantzig, A. H. , F. L. Weigman, Jr. , and A. Nason.	Regulation of glutamate dehydrogenases in nit-2 and am mutants of N. crassa.	1979
Dantzig, A. H. , W. K. Zurowski, T. M. Ball, and A. Nason.	Induction and repression of nitrate reductase in N. crassa.	1978
Facklam, T. J. , and G. A. Marzluf.	Nitrogen regulation of amino acid catabolism in Neurospora crassa.	1978
Fincham, J. R. S.	A comparative genetic study of the mating type chromosomes of two species of Neurospora.	1951
Grove, G. , and G. A. Marzluf.	Indentification of the product of the major regulatory gene of the nitrogen control circuit of Neurosporacrassa as a nuclear DNA-binding protein.	1981
Hanson, M. A. , and G. A. Marzluf.	Control of the synthesis of a single enzyme by multiple regulatory circuits in Neurospora crassa.	1975
Perkins, D. D. , and E. G. Barry.	The cytogenetics of Neurospora.	1977
Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett.	New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage.	1969
Reinert, W. R. , and G. A. Marzluf.	Genetic and metabolic control of the purine catabolic enzymes of Neurospora crassa.	1975
Sorger, G. J. , R. Premakumar, and E. D. Gooden.	Demonstration in vitro of two intracellular inactivators of nitrate reductase fromNeurospora.	1978
Whitehouse, H. L. K.	Crossing-over in Neurospora.	1942
Chambers et al		1983