Gene: nitrate nonutilizer-3 IVR
nitrate nonutilizer-3
locus: nit-3
locus_name: nitrate nonutilizer-3
organism_type: B
chromosome_number: IV
chromosome_side: R
link_group: IVR
cultural_requirements: IVR. Between met-5 (15%) and pyr-2 (2 to 9%) (1000, PB). (453) Cannot use nitrate as a nitrogen source, but uses nitrite, ammonia, hypoxanthine, or amino acids (28, 999). Structural gene for NADPH nitrate reductase (28) (Fig. 19). Allele 14789 apparently codes for an altered enzyme that cannot catalyze the whole electron transport sequence from NADPH to N03-, but can catalyze the terminal portion of this sequence, providing that a suitable electron donor (reduced viologen dye) is provided (999). See reference 198 for a model of interaction of nit-1 and nit-3 gene products. For regulation, see references 226, 999, and 1000. The nit-3+ gene has been cloned and is expressed in Escherichia coli (989).
enzyme_id: 31
Enzyme: NADPH nitrate reductase
anid_name: niaD
near_l: met-5 (15%)
near_r: pyr-2 (2 to 9%)
neu_name:
supplement:
image_url:
Strains
| FGSC # | Reporting Genes | Species |
|---|---|---|
| FGSC #358 | nit-3 | Neurospora crassa |
| FGSC #3009 | nit-3 | Neurospora crassa |
| FGSC #5958 | F. verticillioides nit3 | Fusarium verticillioides |
| FGSC #5966 | F. verticillioides nit3 | Fusarium verticillioides |
| FGSC #6023 | ssu-1;nit-3;inl | Neurospora crassa |
| FGSC #6025 | nit-3 | Neurospora crassa |
| FGSC #6026 | nit-3 | Neurospora crassa |
| FGSC #6027 | nit-3 | Neurospora crassa |
| FGSC #6028 | nit-3 | Neurospora crassa |
| FGSC #6030 | ssu-1 nit-3 | Neurospora crassa |
| FGSC #7209 | cot-1 nit-3 | Neurospora crassa |
| FGSC #7210 | nit-3 pyr-2 | Neurospora crassa |
| FGSC #9545 | nit-3 | Neurospora crassa |
| FGSC #9552 | Not available; nit-3 | Neurospora crassa |
References
