FGSC #863

Mutant Type

Genus: N

reporting_genes: Not available (nd al-2; lys-1 A + pe fl;pan-1 A)

species: Neurospora crassa

allele: (no# 15300.33933) + (Y8743m L;5531)

stock: 1647: Ryan NC 29

glasgow:

mutagen:

Depositor: DDP

Link Group: IR IR;VC + IIR IIR;IVR

MT: A

Species No: 10

gene_back:

oppmt: 0

trans:

ref1: Sheng, T.C. 1951 Genetics 36:199-212 S93, https://www.genetics.org/content/genetics/36/2/199.full.pdf

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-863

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-863 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
pe	IIR. Between nuc-2 (4%) and arg-12 (1 to 5%) (593, 816). (613) Peach-colored conidia and short hyphae formed, more uniformly than by the wild type, as a lawn close to surface of agar. Distinctive morphology (46, 613). Added arginine increases macroconidiation and tends to obscure scoring of peat 25 C, but not at 39°C. pe single mutants produce both macro- and microconidia. pe fl double mutants produce abundant grey microconidia and no macroconidia (46, 700) (see fl). See col-1, col-4, and references 415 and 416 for interactions with other genes. Called m (microconidial) or pem in some contexts.	IIR	B
nd	IR. Between the centromere (15%) and al-2 (20%) (981).Decreasing clonal growth potential under all nutritional conditions, followed by abrupt irreversible cessation of growth (707, 981). Hypersensitive to sorbose. Conidia die rapidly on slants at 4°C (707). Recessive in heterokaryons. An aged strain can be rejuvenated through heterokaryosis or by crossing to nd+. Extracts nontoxic (981). Used to examine hypotheses of senescence based on faulty protein synthesis (607) and lipid autoxidation with free-radical reactions (702). Stocks maintained in balanced heterokaryons. Initial growth rate of the original strain, 2.5 mm/h; however, nd progeny free of modifiers grow initially at 4.5 mm/h (wild-type rate) (707).	IR	B
A			B
lys-1	V. Right of caf-1 (4 to 14%). Left of cyt-9 (5%) and at (1 to 20%) (817; K.S. Hsu, personal communication; PB). (403)Uses lysine, alpha-aminoadipic acid, or epsilon-hydroxynorleucine (alpha-amino-epsilon-hydroxycaproic acid) (399, 400, 684, 1087). Accumulates homocitrate on limiting lysine concentrations (464) (Fig. 16). Fine structure and complementation between alleles (8). Initial allele: 33933.	V	B
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B
fl	IIR. Between ace-1 (5 to 11%) and trp-3 (3%) (816, PB). (613)No macroconidia (609). Highly fertile (612). Used routinely as the female parent in tests for chromosome rearrangements and for mating type (e.g., reference 801). The flsingle mutant produces few microconidia when dry; when wetted, sufficient microconidia are produced to have been used in early irradiation and mutation studies (614, 915); large numbers can be obtained under certain conditions; see reference 893. pe fl (46, 700) and fl;dn (806) double mutants produce abundant microconidia; the latter combination is highly fertile when homozygous. Photograph of microconidial formation (774); see also reference 893. Nuclear numbers in microconidia (46, 64, 478). Wall analysis (207). Immunoelectrophoretic pattern (784). Paradoxical high alcoholic glycolysis on nitrate medium (80). Deficiency of isocitrate lyase on acetate medium; see citations in reference 1088. When fl A and fl a strains are inoculated separately on crossing medium in plates, a double line of perithecia forms where they meet, similar to that accompanying barrage in Podospora (410, 414). fl ascospores from certain fl x fl+ crosses often germinate spontaneously (1127; N. B. Raju, personal communication). Allele C-1835 was called acon (717, 812).	IIR	B
pan-1	IVR. Between ad-6 (1 to 2%) and cot-1 (2 to 3%) (633, 692, PB). (482). cel, col-1, int, pho-3, and thi-5 all appear to be closely linked in this crowded region. Requires intact pantothenic acid for growth under standard conditions. Able to synthesize both precursors, beta-alanine and pantoyl lactone (1058). Ability to synthesize pantothenic acid from beta-alanine plus pantoyl lactone is demonstrable in vitro but not in vivo unless cultures are aerated (1111, 1113, 1114). Unlike pan-2, pan-1 has no effect on ascospore ripening in heterozygous crosses. Called group A. For alleles see reference 138.	IVR	B