FGSC #956

Mutant Type

Genus: N

reporting_genes: al(Y234M468);ad-2

species: Neurospora crassa

allele: Y234M468;Y175M256

stock: 468

glasgow:

mutagen:

Depositor: AMK

Link Group: IR;IIIR

MT: A

Species No: 10

gene_back:

oppmt: 0

trans:

ref1:

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-956

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-956 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ad-2	IIIR. Between thi-2 (1%) and trp-1 (1 to 7%) (11, 219). (482) Requires adenine or hypoxanthine (682). Controls conversion of phosphoribosylformylglycineamidine to AIR (120) (Fig. 8). Strains carrying allele 70004(t) are heat sensitive (34°C versus 25 C) (682) and osmotic remediable (636). Called complementation group H.	IIIR	B
al(Y234M468)	Mutants designated as albino impair carotenoid synthesis. These affect only vegetative cells (mycelia and conidia) and are without known effect on the perithecia or ascospores, where the pigment is melanin. The albino mutants vary in amount and color of carotenoids. Different alleles result in conidia and mycelia that are white, yellow, pink, purple, or white with traces of color or in white mycelia with pigment in the peripheral conidia. See, for example, reference 1042. Carotenoid synthesis is also affected by ylo, wc, and age-3, q.v., and by modifiers of intensity (982). Albino mutants have been used to study the role of carotenoids in photoprotection (984, 1071, and references therein). Rapid development of carotenoids is induced by light; the action spectrum is described in references 250 and 1181, and mechanism of photocontrol is considered in reference 444. However, carotenoid synthesis can proceed slowly in complete darkness. Maximum carotenoid production results if incubation is at 6°C immediately after exposure to inducing light (442). Albino mutants can be scored in submerged colonies from plated ascospores by transfer of sorbose plates to 4 C under light after colonies have grown at 25°C in the dark (154, 500). An unstable constitutive variant has been described (587). Most al mutations map in a short region of IR where al-1 and al-2 were previously thought to be contiguous but are now known to be separated by other loci (797; D.D. Perkins, unpublished data).		B