FGSC #2036

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Reporting Genes: acr-2 dow

Species: crassa

Allele: KH5(r) P616

Alternate Strain Number: 3099

Depositor: DDP

Linkage Group: IIIC IIIR

Mating Type: A

Species Number: 10

Opposite Mating Type: 2037

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
acr-2	III. Linked to thi-4 (0/286). Left of sc (3 to 6%) and spg (1 to 11%) (498, 816). acr-2 has been shown left of the centromere on published maps but without direct evidence. acr-2and trp-1 (on IIIR) cosegregated at the second division in 1 of 13 asci (H.B. Howe, Jr., personal communication), which would favor a right arm location. Resistant to acriflavine (494, 495); also resistant to 3-amino-1,2,4-triazole (seven alleles tested) (494). Resistance is probably dominant (heterokaryon tests) (498). Not resistant to malachite green. An excellent stable marker, fully fertile, with unambiguous scoring. Sizable inocula should be used to avoid false-negative tests. Use acriflavine at 50 µg /ml in minimal agar medium (816) (higher concentrations may be used) and aminotriazole at 0.5 mg/ml; both added before autoclaving.	III	B
dow	IIIR. Right of ty-1 (21%) and un-17 (23%); linked to erg-3 (10%) and acu-7(0/72) (816).Soft, matty growth, conidiating and covering slants (816). An excellent marker: good viability, fertility, and scorability.	IIIR	B