FGSC #10788

Mutant Type

Genus: N

reporting_genes: sar-1::hph, bd

species: Neurospora crassa

allele: sar-1::hph, bd

stock: SP25a

glasgow:

mutagen:

Depositor: Aid

Link Group:

MT: a

Species No: 10

gene_back:

oppmt: 10787

trans:

ref1: Poliano et al 2017 Sci Rep 7:44790, https://doi.org/10.1038/srep44790

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-10788

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-10788 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
bd	Because dense bands of conidia are produced on appropriate solid medium at intervals of about 24 hr (1792, 1794), the mutant has been used extensively to study circadian rhythms (239, 560, 621, 1382, 1794). bd has no effect on the underlying clock mechanism, but allows the visible expression of rhythm (622). Grow rate is about 70% that of the wild type (232). Conidiation is enhanced, even on slants (232). CO2 inhibits conidiation and, thus, inhibits banding; bd is much less sensitive than wild type to this effect of CO2 (1792). Biotin starvation leads to a phenocopy in wild type and to increased persistence of banding in bd (2206). Originally identified in a bd; inv strain called "timex" (1791). bd alone is sufficient to cause banding (1794). Used to study conidiation under nonstarvation conditions (1820). Used in a study of morphological differentiation patterns such as concentric rings and radial zonations (528). Expression is affected by changing the concentrations of agar, sugar, and salts. Conidial scatter is eliminated in the double mutant bd; csp (239). Conveniently scored by conidial banding on agar in long tubes or large plates at 25oC in constant dark or in a dark-light cycle, but not in constant light (1791).	IVR	B
sar-1	I. Near mating type (21). Resistant to surface-active agents dequalinium chloride, cetyltrimethyl ammonium bromide, and benzalkonium chloride. Resistant growth follows an adaptive lag phase. Em A (FGSC 627) and related A laboratory wild types carry a mating-type-linked sar gene that may be sar-1. Another, phenotypically distinct, mutation close to mt is designated sar-3, but evidence for nonallelism is not given (21).	I	B