FGSC #2338

Mutant Type

Genus: N

reporting_genes: tol

species: Neurospora crassa

allele: N83

stock: 4172

glasgow:

mutagen: S

Depositor: DDP

Link Group: IVR

MT: A

Species No: 10

gene_back:

oppmt: 1946

trans:

ref1: Newmeyer 1968. Genetics 60:207 (Abstr)

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-2338

Locus	Cultural Requirements	Link Group	Type
tol	IVR. Linked to trp-4 (~1%), probably to the left (755). Suppresses the vegetative (heterokaryon) incompatibility associated with mating type alleles A and a, but does not affect sexual compatibility. (tol;A+ tol;a) heterokaryons are fully compatible and stable if other het loci are homokaryotic, and A/a duplications grow normally when tol is present (755). Recessive (252); see reference 746, however, for a stable mixed-mating type heterokaryon that is (tol a + tol+ slime A). tol does not suppress the vegetative incompatibility of differing alleles at het-c or het-e(755, 803). Mutation or deletion of tol+ restores normal growth rate to slow-growing, unstable, mixed-mating-type (tol a + tol+ A) heterokaryons (252). tol is present in some isolates from nature and has arisen at least twice by mutation in laboratory stocks (755, PB; O.C. Yoder, personal communication). Double-mutant tol trp-4 stocks are convenient because the closely linked trp-4 tags the tol allele, which otherwise requires progeny tests for scoring. Used to maintain stable A + a heterokaryons, allowing the desired component to be used as the parent in a cross (746). Homozygous tolmay partially restore fertility to the mutant fmf-1 (531).	IVR	B

Neurospora Crassa Wikipedia