FGSC #4146

Mutant Type

Genus: N

reporting_genes: leu-3 ad-3B thi-1 cyh-1

species: Neurospora crassa

allele: R156 2-17-34 56501 KH52(r)

stock: M1253 A

glasgow:

mutagen:

Depositor: EK

Link Group: IL IR

MT: A

Species No: 10

gene_back:

oppmt: 0

trans:

ref1: Kafer 1982. Neurospora Newsl. 29:41-44, https://doi.org/10.4148/1941-4765.1645

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-4146

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-4146 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
leu-3	IL. Right of the In(OY323) left breakpoint and nit-2 (12 to 18%). Left of cyt-1 (5 to 8%) and T(OY321) (57, 816, PB; D.D. Perkins, N.B. Raju, and E.G. Barry, in preparation). (868)Requires leucine (867, 868). Regulatory mutation; prevents synthesis of alpha-isopropylmalate isomerase and beta-isopropylmalate dehydrogenase and prevents full derepression of alpha-isopropylmalate synthetase; also involved in regulation of isoleucine and valine synthesis, q.v. (427, 771, 833) (Fig. 15). The original allele, 47313, is leaky, but some other alleles, e.g., R156, are not.	IL	B
thi-1	IR. Right of the T(4540) right breakpoint and cys-9 (13%). Left of T(NM103), T(ALS182), and met-6 (7 to 14%) (721, 808, 816, 1091). (482). Uses thiamine or precursors pyrimidine plus thiazole (1059). Adaptation to growth on minimal medium occurs after a lag; growth tests should, therefore, be scored early. Adaptation is not carried over via ascospores, conidia, or small mycelial fragments. Adaptive growth is paralleled by attainment of wild-type thiamine pyrophosphate and carboxylase levels. Apparently concerns utilization of intact thiamine rather than its biosynthesis. (302, 303). Allele 17084 is inseparable from translocation T(IR;VII)17084 (808).	IR	B
cyh-1	IR. Right of nit-1 (6%). Left of T(STL76) and al-2 (8 to 13%) (496, 797, 808).Resistant to cycloheximide (496, 748). Resistance is recessive in duplications (1090). Dominance reported in forced heterokaryons (496, 748) may have been due to skewed nuclear ratios (1090). Protein synthesis on ribosomes of the mutant cyh-1 proceeds in the presence of cycloheximide in a cell-free system (834). Readily scored on slants with 10 µg of cycloheximide per ml autoclaved in the medium. Excellent as a marker and valuable for selecting somatic recombinants or deletions in heterozygous duplications (748, 1091). Used to show that the cycloheximide-induced phase shift of the circadian clock involves protein synthesis (738). Called act-1: actidione resistant-1.	IR	B
ad-3B	IR. Between ad-3A (0.1 to 0.7%) and nic-2 (3%) (271). (482) Uses adenine or hypoxanthine (682). Blocked in interconversion of AIR to CAIR (348) (Fig. 8). Produces purple pigment, permitting direct visual selection (276, 682). Pigment is secreted with low concentrations of adenine (e.g., 0.1 mM), not with high concentrations (2 mM) (276, 682, 785). Pigment production used to assess effect of histidine and tryptophan on purine nucleotide synthesis (786). Reduced interallelic fertility (264, 407). Complementation maps (268, 274). Relation of mutagens to complementation patterns (269). Mutants with non-polarized complementation patterns on the right side of the complementation map grow on minimal medium if supplied with CO2; other mutants do not respond to CO2, (270). Used extensively for mutagenesis (see ad-3A). Rearrangement T(I- >III)Y112M4i ad-3B, which has a breakpoint inseparable from ad-3B, was the first insertional translocation to be reported for fungi (266). Allele 7-017-0137 shows "fixed instability," mutating to an unstable prototrophic allele (41). Alleles 2-17-126, 12-21-28, and numerous others are supersuppressible (408, 749, 955). Called complementation group B.	IR	B