FGSC #4548

Mutant Type

Genus: N

reporting_genes: bd;csp-2

species: Neurospora crassa

allele: no#;UCLA101

stock: 9264

glasgow:

mutagen:

Depositor: DDP

Link Group: IVR;VIIL

MT: A

Species No: 10

gene_back:

oppmt: 4549

trans:

ref1:

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-4548

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-4548 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
bd	Because dense bands of conidia are produced on appropriate solid medium at intervals of about 24 hr (1792, 1794), the mutant has been used extensively to study circadian rhythms (239, 560, 621, 1382, 1794). bd has no effect on the underlying clock mechanism, but allows the visible expression of rhythm (622). Grow rate is about 70% that of the wild type (232). Conidiation is enhanced, even on slants (232). CO2 inhibits conidiation and, thus, inhibits banding; bd is much less sensitive than wild type to this effect of CO2 (1792). Biotin starvation leads to a phenocopy in wild type and to increased persistence of banding in bd (2206). Originally identified in a bd; inv strain called "timex" (1791). bd alone is sufficient to cause banding (1794). Used to study conidiation under nonstarvation conditions (1820). Used in a study of morphological differentiation patterns such as concentric rings and radial zonations (528). Expression is affected by changing the concentrations of agar, sugar, and salts. Conidial scatter is eliminated in the double mutant bd; csp (239). Conveniently scored by conidial banding on agar in long tubes or large plates at 25oC in constant dark or in a dark-light cycle, but not in constant light (1791).	IVR	B
csp-2	VIIL. Linked to thi-3 (<1%), probably to the right. Left of T(T54M40) (972, PB). Conidia fail to separate and become airborne. Cultures on agar readily scored by the tap test. Resembles csp-1. Conidia are freed in water suspension long after induction of aerial growth and at only 1/100 the concentration of the wild type. A csp-1;csp-2double mutant releases no detectable free conidia under the same conditions (972). Most csp-2alleles complement csp-1 in forced heterokaryons to form the wild-type number of free conidia (972), but csp-2(UCLA102) does not (969). Conidiating colonies of the csp-2;sn cr-1 strain on replica plates can be overlayered without the conidia being spread (744); photograph (747).	VIIL	B