FGSC #4633

Mutant Type

Genus: N

reporting_genes: This strain is not available T(IIL;VL)AR30 T(IIR;VR)ALS154 arg-5 fl ilv acr-3

species: Neurospora crassa

allele: AR30 ALS154 27947 P STL3 KH24(r)

stock: 156M10

glasgow:

mutagen:

Depositor: JFL

Link Group: VL VR;IL;IIL IIR

MT: A

Species No: 10

gene_back:

oppmt: 4634

trans:

ref1: https://doi.org/10.1007/BF02424417

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-4633

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-4633 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
T(IIL;VL)AR30	Translocation		B
fl	IIR. Between ace-1 (5 to 11%) and trp-3 (3%) (816, PB). (613)No macroconidia (609). Highly fertile (612). Used routinely as the female parent in tests for chromosome rearrangements and for mating type (e.g., reference 801). The flsingle mutant produces few microconidia when dry; when wetted, sufficient microconidia are produced to have been used in early irradiation and mutation studies (614, 915); large numbers can be obtained under certain conditions; see reference 893. pe fl (46, 700) and fl;dn (806) double mutants produce abundant microconidia; the latter combination is highly fertile when homozygous. Photograph of microconidial formation (774); see also reference 893. Nuclear numbers in microconidia (46, 64, 478). Wall analysis (207). Immunoelectrophoretic pattern (784). Paradoxical high alcoholic glycolysis on nitrate medium (80). Deficiency of isocitrate lyase on acetate medium; see citations in reference 1088. When fl A and fl a strains are inoculated separately on crossing medium in plates, a double line of perithecia forms where they meet, similar to that accompanying barrage in Podospora (410, 414). fl ascospores from certain fl x fl+ crosses often germinate spontaneously (1127; N. B. Raju, personal communication). Allele C-1835 was called acon (717, 812).	IIR	B
T(IIR;VR)ALS154	Translocation		B
acr-3	IL. Between un-16 (1 to 5%) and suc (1 to 5%). Probably right of ta (816; PB). (498)Resistant to acriflavine and to malachite green (three alleles tested). Not resistant to 3-aminotriazole. Resistance is probably dominant (heterokaryon tests) (498). Scoring is clear-cut with uniform inocula of appropriate size. False-negative or false-positive scoring may result if test inocula are too small or too large. May show delayed resistance: read tests at 2 and 4 days, 34°C. Use acriflavine at 10 µg /ml in minimal agar at 34°C (816) and malachite green at 2 µg /ml (498).	IL	B
ilv	Three loci specify enzymes that catalyze corresponding steps in the parallel biosynthetic pathways of isoleucine and valine (Fig. 15). These enzymes are located in the mitochondria (79, 597 and references therein) and may indirectly affect electron transport (79). The enzymes may be in an aggregate; for a review, see reference 237. The requirements are for both amino acids. A ratio of 20-30% isoleucine to 80-70% valine is optimal (99). At least some ilv mutants are inhibited by norleucine, norvaline, phenylalanine (99), or tryptophan (J.F. Leslie, personal communication). Enzyme production in response to end-product-derived signals depends on the leu-3+ product and alpha-isopropylmalate. In leu-3+ strains, threonine deaminase production is repressed as a function of available isoleucine, acetohydroxyacid synthetase is repressed as a function of valine, and isomeroreductase and dihydroxyacid dehydratase are repressed as a function of isoleucine and leucine. In the absence of effective leu-3product, alpha-IPM, or both, enzyme production is repressed even under severe end product limitation. (771) Formerly called iv.Biosynthetic pathways of isoleucine, valine, and leucine, showing sites of gene action (22, 136. 426, 549, 727. 854, 1112). Isoleucine and valine are synthesized along parallel pathways catalyzed by common enzymes. The leucine precursors alpha- and beta-isopropylmalate were formerly called beta-OH-beta-carboxyisocaproate and alpha-OH-beta-carboxyisocaproate, respectively.		B
arg-5	Uses ornithine, citrulline or arginine.	IIR	B