FGSC #4810

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Reporting Genes: pmb;pmg

Species: crassa

Allele: can-37(r);no#

Alternate Strain Number: FGSC 662

Depositor: RMD

Linkage Group: IVR;IIL

Mating Type: A

Species Number: 10

Genetic Background: SL

Opposite Mating Type: 4811

ref1: DeBusk and DeBusk J. Bact. 143:188-197, https://doi.org/10.1128/jb.143.1.188-197.1980

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
pmb	IVR. Right of uvs-2 (8%) (S. Ogilvie-Villa, cited in reference 248; R. Sadler and S. Ogilvie-Villa, personal communication). Defective in basic L-amino acid transport (system III as defined in reference 778); reduced uptake of L-arginine, L-lysine, and L-histidine (778, 1152, 1076). Used extensively for transport studies; see reference 1150. Altered surface glycoprotein (1038). pmb mutants selected as resistant to L-canavanine (889, 1152). Allelic with bat (R. Sadler and S. Ogilvie-Villa, personal communication), which was selected in arg-12s;pyr-3 (CPS- ACT+) by ability to grow on minimal medium plus arginine, when the parental double mutant was not able to grow because of arginine uptake and feedback onto the arginine-specific carbamyl phosphate synthase (1074). Possibly allelic with basa, which was selected by the inability of the mutant his-3 to grow on histidine plus methionine (628). Probably allelic with bm-1 (linked to pyr-2, 24%), which was selected by canavanine resistance (913). Probably allelic with argR (565, 566), q.v. Called Cr-10, Pm-B, pm b, UM-535, and can-37. See Transport.	IVR	B
pmg	IIL. Linked to pyr-4. Right of ro-3 (4%) (29%). Centromere linked. Not linked to mtr(pmn) or to pmb in IVR (248). (Previous report of mating type linkage [862] not confirmed). Greatly reduced in general amino acid transport system II (as defined in reference 777). Reduced uptake of arginine and phenylalanine. Selected by resistance to p-fluorophenylalanine in a neutral (system I), basic (system III) double mutant on medium lacking ammonium ions, where system II would be derepressed in the wild type (248, 862, 863). A non-metabolizable substrate specific for this transport system has been found (767). Called pm g. See Transport.	IIL	B