FGSC #4901

Mutant Type

Genus: N

reporting_genes: bd;frq-8

species: Neurospora crassa

allele: no#;no#

stock: 600-45

glasgow:

mutagen:

Depositor: JFF

Link Group: IVR;VIIR

MT: a

Species No: 10

gene_back: SL

oppmt: 4900

trans:

ref1: Gardner & Feldman 1980. Genetics 96:877-886, https://doi.org/10.1093/genetics/96.4.877

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-4901

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-4901 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
bd	Because dense bands of conidia are produced on appropriate solid medium at intervals of about 24 hr (1792, 1794), the mutant has been used extensively to study circadian rhythms (239, 560, 621, 1382, 1794). bd has no effect on the underlying clock mechanism, but allows the visible expression of rhythm (622). Grow rate is about 70% that of the wild type (232). Conidiation is enhanced, even on slants (232). CO2 inhibits conidiation and, thus, inhibits banding; bd is much less sensitive than wild type to this effect of CO2 (1792). Biotin starvation leads to a phenocopy in wild type and to increased persistence of banding in bd (2206). Originally identified in a bd; inv strain called "timex" (1791). bd alone is sufficient to cause banding (1794). Used to study conidiation under nonstarvation conditions (1820). Used in a study of morphological differentiation patterns such as concentric rings and radial zonations (528). Expression is affected by changing the concentrations of agar, sugar, and salts. Conidial scatter is eliminated in the double mutant bd; csp (239). Conveniently scored by conidial banding on agar in long tubes or large plates at 25oC in constant dark or in a dark-light cycle, but not in constant light (1791).	IVR	B
frq-8	VIIR. Between un-10 (9%) and for (3%) (J.F. Feldman, personal communication). Right of met-9 (9 to 13%). Linked to oli (< 2%); possibly allelic (282). (330)A series of clustered genes or of multiple alleles resulting in altered periods in the circadian rhythm cycle of conidiation. Identified mutations and their periods (at 25 C without csp) are: frq-1, 16.5 h; frq-2,19.3 h; frq-3, 24.0 h; and frq-4, 19.3 h (331); frq-6, 19.2 h; frq-7, 29.0 h; and frq-8, 29.0 h (wild type, 21.5 h) (329, 377). Strains carrying allele UV-111-9 show erratic periodicity. Temperature compensation and interactions with other loci have been described; dominance is incomplete; growth rates are normal (326, 375-377). Scoring is accomplished by zonation in growth tubes or plates, using strains that carry bd and preferably csp-1 or csp-2. Presence of csp shortened period length about 1 h in the one strain tested (279). Period-altering mutations elsewhere in the genome are given different names (e.g., prd and chr). The symbol frq is reserved for this locus or region (329). For review of circadian mutants, see references 326 and 328.		B