FGSC #7322

Mutant Type

Genus: N

reporting_genes: leu-3 suc;tol pan-1

species: Neurospora crassa

allele: R156 66702;N83 5531

stock: 9230

glasgow:

mutagen:

Depositor: DDP

Link Group: IL IL;IVR IVR

MT: a

Species No: 10

gene_back:

oppmt: 0

trans:

ref1:

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-7322

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-7322 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
leu-3	IL. Right of the In(OY323) left breakpoint and nit-2 (12 to 18%). Left of cyt-1 (5 to 8%) and T(OY321) (57, 816, PB; D.D. Perkins, N.B. Raju, and E.G. Barry, in preparation). (868)Requires leucine (867, 868). Regulatory mutation; prevents synthesis of alpha-isopropylmalate isomerase and beta-isopropylmalate dehydrogenase and prevents full derepression of alpha-isopropylmalate synthetase; also involved in regulation of isoleucine and valine synthesis, q.v. (427, 771, 833) (Fig. 15). The original allele, 47313, is leaky, but some other alleles, e.g., R156, are not.	IL	B
pan-1	IVR. Between ad-6 (1 to 2%) and cot-1 (2 to 3%) (633, 692, PB). (482). cel, col-1, int, pho-3, and thi-5 all appear to be closely linked in this crowded region. Requires intact pantothenic acid for growth under standard conditions. Able to synthesize both precursors, beta-alanine and pantoyl lactone (1058). Ability to synthesize pantothenic acid from beta-alanine plus pantoyl lactone is demonstrable in vitro but not in vivo unless cultures are aerated (1111, 1113, 1114). Unlike pan-2, pan-1 has no effect on ascospore ripening in heterozygous crosses. Called group A. For alleles see reference 138.	IVR	B
suc	IL. Right of acr-3 (2%). Left of In(H4250) and phe-1(1%) (578, 816). (482). Uses acetate, succinate, or any of numerous related compounds (608). Most but not all strains grow better on acetate than on succinate (576). Lacks pyruvate carboxylase activity (72, 578, 1032). Numerous alleles are CO2 remediable (108). Leaky, but less so on higher ammonium concentrations (1031). A special medium has been devised for selective plating (K.D. Munkres, cited in reference 816, p. 248). Most ascospores are poorly pigmented in homozygous suc x succrosses. Allele KG163 shows greatly reduced recombination in the region between leu-4 and suc, suggesting inversion heterozygosity (576). suc would be named ace-6 except for priority of nomenclature (578).	IL	B
tol	IVR. Linked to trp-4 (~1%), probably to the left (755). Suppresses the vegetative (heterokaryon) incompatibility associated with mating type alleles A and a, but does not affect sexual compatibility. (tol;A+ tol;a) heterokaryons are fully compatible and stable if other het loci are homokaryotic, and A/a duplications grow normally when tol is present (755). Recessive (252); see reference 746, however, for a stable mixed-mating type heterokaryon that is (tol a + tol+ slime A). tol does not suppress the vegetative incompatibility of differing alleles at het-c or het-e(755, 803). Mutation or deletion of tol+ restores normal growth rate to slow-growing, unstable, mixed-mating-type (tol a + tol+ A) heterokaryons (252). tol is present in some isolates from nature and has arisen at least twice by mutation in laboratory stocks (755, PB; O.C. Yoder, personal communication). Double-mutant tol trp-4 stocks are convenient because the closely linked trp-4 tags the tol allele, which otherwise requires progeny tests for scoring. Used to maintain stable A + a heterokaryons, allowing the desired component to be used as the parent in a cross (746). Homozygous tolmay partially restore fertility to the mutant fmf-1 (531).	IVR	B