FGSC #7337

Mutant Type

Genus: N

reporting_genes: leu-3 cyt-1 arg-3;tol

species: Neurospora crassa

allele: R156 C115 30300;N83

stock: 9259

glasgow:

mutagen:

Depositor: DDP

Link Group: IL L L;IVR

MT: A

Species No: 10

gene_back:

oppmt: 0

trans:

ref1:

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-7337

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-7337 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
cyt-1	IL. Between leu-3 (5 to 8%) (583) and T(OY321) (D.D. Perkins, N.B. Raju, and E.G. Barry, in preparation). (694) Deficient in cytochromes aa3 and b. Very slow growth. Female sterile (87, 694). Scoring aided by slower growth on complete medium relative to that on minimal, presumably owing to inhibition by yeast extract (694, 816). cyt-U-9 is linked and may be allelic with the original cyt-1 mutation C115 (87; H. Bertrand, personal communication).	IL	B
leu-3	IL. Right of the In(OY323) left breakpoint and nit-2 (12 to 18%). Left of cyt-1 (5 to 8%) and T(OY321) (57, 816, PB; D.D. Perkins, N.B. Raju, and E.G. Barry, in preparation). (868)Requires leucine (867, 868). Regulatory mutation; prevents synthesis of alpha-isopropylmalate isomerase and beta-isopropylmalate dehydrogenase and prevents full derepression of alpha-isopropylmalate synthetase; also involved in regulation of isoleucine and valine synthesis, q.v. (427, 771, 833) (Fig. 15). The original allele, 47313, is leaky, but some other alleles, e.g., R156, are not.	IL	B
tol	IVR. Linked to trp-4 (~1%), probably to the left (755). Suppresses the vegetative (heterokaryon) incompatibility associated with mating type alleles A and a, but does not affect sexual compatibility. (tol;A+ tol;a) heterokaryons are fully compatible and stable if other het loci are homokaryotic, and A/a duplications grow normally when tol is present (755). Recessive (252); see reference 746, however, for a stable mixed-mating type heterokaryon that is (tol a + tol+ slime A). tol does not suppress the vegetative incompatibility of differing alleles at het-c or het-e(755, 803). Mutation or deletion of tol+ restores normal growth rate to slow-growing, unstable, mixed-mating-type (tol a + tol+ A) heterokaryons (252). tol is present in some isolates from nature and has arisen at least twice by mutation in laboratory stocks (755, PB; O.C. Yoder, personal communication). Double-mutant tol trp-4 stocks are convenient because the closely linked trp-4 tags the tol allele, which otherwise requires progeny tests for scoring. Used to maintain stable A + a heterokaryons, allowing the desired component to be used as the parent in a cross (746). Homozygous tolmay partially restore fertility to the mutant fmf-1 (531).	IVR	B
arg-3	Uses citrulline or arginine.	IL	B