FGSC #7387

Mutant Type

Genus: N

reporting_genes: Sk-2K acr-2 leu-1 his-7

species: Neurospora crassa

allele: Borneo KH5 33757 Y152M31

stock: 3273

glasgow:

mutagen:

Depositor: BCT

Link Group: III III III III

MT: A

Species No: 10

gene_back:

oppmt: 7388

trans:

ref1:

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-7387

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-7387 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
his-7	IIIR. Between leu-1 (8 to 20%) and thi-2 (1 to 2%) (2199 1052, PB). (162, 1123)Requires histidine. Blocked before imidazole glycerol phosphate (162, 1123) (Fig. 14). Intralocus recombination (172).	IIIR	B
leu-1	IIIR. Between ad-4 (1 to 5%) and his-7 (8%) (219, 578, 815, 1052). (868)Requires leucine (867, 868). Lacks beta-isopropylmalate dehydrogenase (426) (Fig. 15). Accumulates alpha-isopropylmalate and beta-isopropylmalate (427). Synthesis of the enzyme also requires the function of regulatory gene leu-3+ and the presence of alpha-isopropylmalate, which acts as inducer (427). Resistant to aminotriazole (D. D. Perkins, unpublished data). Female sterile (O. M. Mylyk, personal communication). Used to study reversion and competition in heterokaryons (901).	IIIR	B
acr-2	III. Linked to thi-4 (0/286). Left of sc (3 to 6%) and spg (1 to 11%) (498, 816). acr-2 has been shown left of the centromere on published maps but without direct evidence. acr-2and trp-1 (on IIIR) cosegregated at the second division in 1 of 13 asci (H.B. Howe, Jr., personal communication), which would favor a right arm location. Resistant to acriflavine (494, 495); also resistant to 3-amino-1,2,4-triazole (seven alleles tested) (494). Resistance is probably dominant (heterokaryon tests) (498). Not resistant to malachite green. An excellent stable marker, fully fertile, with unambiguous scoring. Sizable inocula should be used to avoid false-negative tests. Use acriflavine at 50 µg /ml in minimal agar medium (816) (higher concentrations may be used) and aminotriazole at 0.5 mg/ml; both added before autoclaving.	III	B
Sk-2K			B