enhancer-1 of am

locus: en(am)-1

locus_name: enhancer-1 of am

organism_type: B

chromosome_number: V

chromosome_side: R

link_group: VR

cultural_requirements: VR. Between am (8%) and inl (1%). Linked to gln(1%). (122, 339, 345)In en(am)-1 am double mutants, en(am)-1 blocks the adaptation of am on minimal medium without a source of amino nitrogen (345). The double mutants are inhibited by ammonium and grow adequately only when glutamate is the sole nitrogen source. The en(am)-1single mutant grows well on minimal medium, but is unable to use, as the sole nitrogen source, proline (122), methionine, alanine, isoleucine, valine, urocanate, hypoxanthine, uridine, urea, or bovine serum albumin (184). It is relatively resistant to p-fluorophenylalanine and ethionine and completely resistant to 0.02 M glycine. These properties cosegregated with en(am)-1 in all isolates tested (339). Glutamate synthase (GOGAT) is normal (293). The single mutant is scored by using minimal medium with proline as the sole nitrogen source (122) or (better) by using 0.2 mM p-fluorophenylalanine (339). Name changed from i (inhibitor) (293).

enzyme_id: 202

Enzyme: none

anid_name:

near_l: am (8%)

near_r: inl (1%). Linked to gln(1%)

neu_name:

supplement:

image_url:

Strains

FGSC #	Reporting Genes	Species
FGSC #3963	en(am)-1	Neurospora crassa
FGSC #3964	en(am)-1	Neurospora crassa

References

Authors	Title	Year
Burk, R. R.	The location of i, enhancer of am.	1964
Chambers, J. A. A.	The i phenotype of N. crassa.	1980
Dunn-Coleman, N. S. , E. A. Robey, A. B. Tomsett, and R. H. Garrett.	Glutamate synthase levels in Neurospora crassa mutants alteredwith respect to nitrogen metabolism.	1981
Fincham, J. R. S.	Further properties of the i (en-am-1) mutant.	1981
Fincham, J. R. S. , and J. A. Pateman.	A new allele at the am locus in Neurospora crassa.	1957
Chambers et al		1983