FGSC #3963

Mutant Type

Genus: N

reporting_genes: en(am)-1

species: Neurospora crassa

allele: no#

stock: 2i

glasgow:

mutagen:

Depositor: JRF

Link Group: VR

MT: a

Species No: 10

gene_back:

oppmt: 0

trans:

ref1: Fincham 1981 Neurospora Newsl 28:11, https://doi.org/10.4148/1941-4765.1651

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-3963

Locus	Cultural Requirements	Link Group	Type
en(am)-1	VR. Between am (8%) and inl (1%). Linked to gln(1%). (122, 339, 345)In en(am)-1 am double mutants, en(am)-1 blocks the adaptation of am on minimal medium without a source of amino nitrogen (345). The double mutants are inhibited by ammonium and grow adequately only when glutamate is the sole nitrogen source. The en(am)-1single mutant grows well on minimal medium, but is unable to use, as the sole nitrogen source, proline (122), methionine, alanine, isoleucine, valine, urocanate, hypoxanthine, uridine, urea, or bovine serum albumin (184). It is relatively resistant to p-fluorophenylalanine and ethionine and completely resistant to 0.02 M glycine. These properties cosegregated with en(am)-1 in all isolates tested (339). Glutamate synthase (GOGAT) is normal (293). The single mutant is scored by using minimal medium with proline as the sole nitrogen source (122) or (better) by using 0.2 mM p-fluorophenylalanine (339). Name changed from i (inhibitor) (293).	VR	B

Neurospora Crassa Wikipedia