methyltryptophan sensitive

locus: mts

locus_name: methyltryptophan sensitive

organism_type: B

chromosome_number: VI

chromosome_side: L

link_group: VIL

cultural_requirements: VIL. Right of ylo-1 (<1%) (152, 160). Sensitive to analogs of all tested aromatic, neutral, and basic amino acids and to analogs of purines and pyrimidines. Ten to 100 times more sensitive than the wild type. Not sensitive to cold, salt, or detergent. Resembles mod-5 in enabling the mutant trp-3(A78) to grow well on complex media, but differs in not doing so for the mutant pyr-1(H263). No allelism test with mod-5. Obtained by filtration enrichment in the presence of 5-methyltryptophan (152, 160). Used for selection of mutants resistant to analogs: 5-methyltryptophan (152); 8-azaadenine (524). Could be useful where the wild type is not sufficiently sensitive to allow direct selection of resistant mutants. Conveniently scored on p-fluorophenylalanine (2 µg/ml, solid medium, autoclaved in medium; p-fluorophenylalanine is more heat stable than is 5-methyltryptophan). Best tested with small inocula on slants (10 by 75 mm) and read after 3 days at 34°C (PB). Called 5mt.

enzyme_id:

anid_name:

near_l: ylo-1 (<1%)

near_r:

neu_name:

supplement:

image_url:

Strains

FGSC #	Reporting Genes	Species
FGSC #2746	Has been replaced by strain number: 4379	Neurospora crassa
FGSC #2747	Has been replaced by strain number: 4380	Neurospora crassa
FGSC #4379	mts	Neurospora crassa
FGSC #4380	mts	Neurospora crassa
FGSC #6699	T(IVR;VIL)MN9 mts	Neurospora crassa
FGSC #6700	T(IVR;VIL)MN9 mts	Neurospora crassa

References

Authors	Title	Year
Catcheside, D. E. A.	Genetic and biochemical studies in Neurospora crassa.	1966
Catcheside, D. E. A.	A pleiotropic mutation in Neurospora conferring sensitivity to analogues of amino acids, purines andpyrimidines.	1978
Jha, K. K.	Genes conferring resistance to 8-aza adenine in Neurospora crassa and the variability of resistant alleles in the aza-1 locuswith respect to excretion of purines.	1971