FGSC #4379

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Reporting Genes: mts

Species: crassa

Allele: MN1

Alternate Strain Number: 6154

Mutagen: UV

Depositor: DDP

Linkage Group: VI

Mating Type: A

Species Number: 10

Genetic Background: SL2

Opposite Mating Type: 4380

ref1: NN 25:17-18, FGN 38:71

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
mts	VIL. Right of ylo-1 (<1%) (152, 160). Sensitive to analogs of all tested aromatic, neutral, and basic amino acids and to analogs of purines and pyrimidines. Ten to 100 times more sensitive than the wild type. Not sensitive to cold, salt, or detergent. Resembles mod-5 in enabling the mutant trp-3(A78) to grow well on complex media, but differs in not doing so for the mutant pyr-1(H263). No allelism test with mod-5. Obtained by filtration enrichment in the presence of 5-methyltryptophan (152, 160). Used for selection of mutants resistant to analogs: 5-methyltryptophan (152); 8-azaadenine (524). Could be useful where the wild type is not sufficiently sensitive to allow direct selection of resistant mutants. Conveniently scored on p-fluorophenylalanine (2 µg/ml, solid medium, autoclaved in medium; p-fluorophenylalanine is more heat stable than is 5-methyltryptophan). Best tested with small inocula on slants (10 by 75 mm) and read after 3 days at 34°C (PB). Called 5mt.	VIL	B