Gene: nitrate nonutilizer-1 IR

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nitrate nonutilizer-1

locus: nit-1

locus_name: nitrate nonutilizer-1

organism_type: B

chromosome_number: I

chromosome_side: R

link_group: IR

cultural_requirements: IR. Right of Tp(T54M94) and ad-9 (3 to 15%). Left of cyh-1(6%) (466, 496, 816). (482)Cannot use nitrate or hypoxanthine as a nitrogen source, but uses nitrite, ammonia, or amino acids (1000). Does not prevent formation or nitrate reductase apoprotein (999), but lacks the molybdenum-containing cofactor common to nitrate reductase and xanthine dehydrogenase (591, 741) (Fig. 19 and 24). The nitrate reductase in nit-1 extracts does not catalyze the complete electron transport sequence from NADPH to N03 but does catalyze the initial part of this sequence if a suitable electron acceptor (e.g., cytochrome c) is provided (999). See reference 198 for a model of interaction of nit-1 and nit-3 gene products. See references 226, 999, and 1000 for regulation.

enzyme_id: 137

Enzyme: molybdenum cofactor - nitrate reductase and xanthine dehydrogenase

anid_name: cnxABC

near_l: Tp(T54M94) and ad-9 (3 to 15%)

near_r: cyh-1(6%)

neu_name:

supplement:

image_url:

Strains

FGSC # Reporting Genes Species
FGSC #32nit-1Neurospora crassa
FGSC #54nit-1Neurospora crassa
FGSC #228nit-1 al-1 nic-1 os-1Neurospora crassa
FGSC #236cr-1 thi-1 nit-1 al-1 nic-1 os-1;cot-1Neurospora crassa
FGSC #245cr-1 thi-1 nit-1 al-1 nic-1 os-1Neurospora crassa
FGSC #258cr-1 nit-1 al-1 os-1Neurospora crassa
FGSC #288cr-1 nit-1 al-1 os-1Neurospora crassa
FGSC #5955F. verticillioides nit1Fusarium verticillioides
FGSC #5956F. verticillioides nit1Fusarium verticillioides
FGSC #7348thi-1 ad-9 nit-1 het-5PANeurospora crassa
FGSC #7349thi-1 ad-9 nit-1 het-5PANeurospora crassa

References

Authors Title Year
Holm, K. , E. Nilheden, H. G. Kolmark, and K. Burton.Genetic and enzymatic analysis of a glycerol kinase deficient mutant inNeurospora crassa.1976
Houlahan, M. B. , G. W. Beadle, and H. G. Calhoun.Linkage studies with biochemical mutants of Neurospora crassa.1949
Hsu, K. S.The genetic basis of actidione resistance in Neurospora.1963
Lee, K. -Y. , S. -S. Pan, R. Erickson, and A. Nason.Involvement of molybdenum and iron in the in vitro assembly of assimilatory nitratereductase utilizing Neurospora mutant nit-1 .1974
Nason, A. , K. -Y. Lee, S. -S. Pan, P. A. Ketchum, A. Lamberti, and J. De Vries.In vitro formation of assimilatory reduced nicotinamideadenine dinucleotide phosphate:nitrate reductase from a Neurospora mutant and a component of molybdenum-enzymes.1971
Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett.New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage.1969
Sorger, G. J.Nitrate reductase electron transport systems in mutant and in wild-type strains of Neurospora.1966
Sorger, G. J. , and N. H. Giles.Genetic control of nitrate reductase in Neurospora crassa.1965

Neurospora Crassa Wikipedia

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