FGSC #7349

Mutant Type

Genus: N

reporting_genes: thi-1 ad-9 nit-1 het-5PA

species: Neurospora crassa

allele: 56501 Y154M37 34547 PA

stock: DJ947-25a

glasgow:

mutagen:

Depositor: DJJ

Link Group: IR

MT: a

Species No: 10

gene_back: SL9

oppmt: 7348

trans:

ref1: Perkins, Leslie and Jacobson 1993 FGN 40, https://doi.org/10.4148/1941-4765.1419

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-7349

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-7349 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ad-9	IR. Right of met-6 (9 to 16%). Left of Tp(T54M94) and nit-1 (3 to 15%) (466, 816). (815) Uses adenine or hypoxanthine (526). Controls conversion of phosphoribosylglycineamide to phosphoribosylformylglycineamide (120) (Fig. 8). Called complementation group D.	IR	B
het-5PA	IR. Between T(NM103) and the tip; hence, right of thi-1 (729).Vegetative incompatibility recognized by inhibited duplications and subsequently confirmed by heterokaryon tests (729, 730).	IR	B
nit-1	IR. Right of Tp(T54M94) and ad-9 (3 to 15%). Left of cyh-1(6%) (466, 496, 816). (482)Cannot use nitrate or hypoxanthine as a nitrogen source, but uses nitrite, ammonia, or amino acids (1000). Does not prevent formation or nitrate reductase apoprotein (999), but lacks the molybdenum-containing cofactor common to nitrate reductase and xanthine dehydrogenase (591, 741) (Fig. 19 and 24). The nitrate reductase in nit-1 extracts does not catalyze the complete electron transport sequence from NADPH to N03 but does catalyze the initial part of this sequence if a suitable electron acceptor (e.g., cytochrome c) is provided (999). See reference 198 for a model of interaction of nit-1 and nit-3 gene products. See references 226, 999, and 1000 for regulation.	IR	B
thi-1	IR. Right of the T(4540) right breakpoint and cys-9 (13%). Left of T(NM103), T(ALS182), and met-6 (7 to 14%) (721, 808, 816, 1091). (482). Uses thiamine or precursors pyrimidine plus thiazole (1059). Adaptation to growth on minimal medium occurs after a lag; growth tests should, therefore, be scored early. Adaptation is not carried over via ascospores, conidia, or small mycelial fragments. Adaptive growth is paralleled by attainment of wild-type thiamine pyrophosphate and carboxylase levels. Apparently concerns utilization of intact thiamine rather than its biosynthesis. (302, 303). Allele 17084 is inseparable from translocation T(IR;VII)17084 (808).	IR	B