Gene: nuclease-1 IR
nuclease-1
locus: nuc-1
locus_name: nuclease-1
organism_type: B
chromosome_number: I
chromosome_side: R
link_group: IR
cultural_requirements: IR. Right of T(AR173) and his-2 (<1%). Left of lys-4(1%). (514) nuc-1mutants (other than nuc-1c) are unable to use RNA or DNA as a phosphorus source (450, 514). Defective in production of repressible alkaline and acid phosphatases (671, 1077). Several nucleases are absent or reduced (449). nuc-1 is epistatic to both pconc and pregc (671) and to pgovc (665). Scored on low-phosphate medium by a staining reaction with alpha-naphthyl phosphate plus diazo blue B (397, 1077), by failure to grow on minimal medium altered so that 0.1 g of RNA or DNA per liter is substituted for the inorganic phosphate source (514, 538), or by failure to grow on low-phosphate medium at a pH above 7 (R.L. Metzenberg, personal communication). nuc-1c is constitutive for alkaline phosphatase synthesis and maps very close to nuc-1. nuc-1c acts only if it is cis to normal nuc-1. In duplications, nuc-1c is dominant to nuc-1+, which is dominant to nuc-1. nuc-1c is epistatic to nuc-2(670). nuc-1c is scored on high-phosphate medium by a staining reaction with alpha-naphthyl phosphate plus diazo blue B (397, 1077) or by suppression of the nuc-2 phenotype on low-phosphate medium at high pH (670). Used to study phosphate transport (624). For regulation model see references 665 and 670.
enzyme_id: 59
Enzyme: alkaline phosphatase acid phosphatase
anid_name: palcA?
near_l: T(AR173) and his-2 (<1%)
near_r: lys-4(1%)
neu_name:
supplement:
image_url:
Strains
| FGSC # | Reporting Genes | Species |
|---|---|---|
| FGSC #1994 | nuc-1 | Neurospora crassa |
| FGSC #1995 | nuc-1 | Neurospora crassa |
| FGSC #1998 | nuc-1;nuc-2 | Neurospora crassa |
| FGSC #1999 | nuc-1 ad-3A | Neurospora crassa |
| FGSC #3480 | pgovc;nuc-1 | Neurospora crassa |
References
