FGSC #1995

Reporting Genes: nuc-1

Species: crassa

Allele: T28-M1 (Also A1)

Mutagen: UV

Depositor: TI

Linkage Group: IR

Mating Type: A

Species Number: 10

Genetic Background: M

ref1: Ishikawa et al. 1969. Genetics 63:75-92, https://www.genetics.org/content/genetics/63/1/75.full.pdf

ref2: Uno and Ishikawa 1969. Mutation Res. 8:239-246, https://doi.org/10.1016/0027-5107(69)90003-7

Genes

Locus	Cultural Requirements	Link Group	Type
nuc-1	IR. Right of T(AR173) and his-2 (<1%). Left of lys-4(1%). (514) nuc-1mutants (other than nuc-1c) are unable to use RNA or DNA as a phosphorus source (450, 514). Defective in production of repressible alkaline and acid phosphatases (671, 1077). Several nucleases are absent or reduced (449). nuc-1 is epistatic to both pconc and pregc (671) and to pgovc (665). Scored on low-phosphate medium by a staining reaction with alpha-naphthyl phosphate plus diazo blue B (397, 1077), by failure to grow on minimal medium altered so that 0.1 g of RNA or DNA per liter is substituted for the inorganic phosphate source (514, 538), or by failure to grow on low-phosphate medium at a pH above 7 (R.L. Metzenberg, personal communication). nuc-1c is constitutive for alkaline phosphatase synthesis and maps very close to nuc-1. nuc-1c acts only if it is cis to normal nuc-1. In duplications, nuc-1c is dominant to nuc-1+, which is dominant to nuc-1. nuc-1c is epistatic to nuc-2(670). nuc-1c is scored on high-phosphate medium by a staining reaction with alpha-naphthyl phosphate plus diazo blue B (397, 1077) or by suppression of the nuc-2 phenotype on low-phosphate medium at high pH (670). Used to study phosphate transport (624). For regulation model see references 665 and 670.	IR	B

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