phenylalanine-2

locus: phe-2

locus_name: phenylalanine-2

organism_type: B

chromosome_number: III

chromosome_side: R

link_group: IIIR

cultural_requirements: IIIR. Linked to vel (1%), between T(D305) and tyr-1(2 to 4%) (40, 316, 809). Lacks prephenic dehydratase (40, 316) (Fig. 11). Requirement is very leaky. Grows extensively and is treacherous to score by growth on minimal versus supplemented medium, but can be scored reliably by blue fluorescence under long-wave UV after growth on minimal medium without phenylalanine (816). Appearance of phenylalanine in culture medium (118) is due to spontaneous conversion of accumulated pretyrosine (519). Called phen-2. Allele Y16329 formerly called phen-3 (316).

enzyme_id: 162

Enzyme: prephenic dehydratase

anid_name: phenA

near_l: T(D305)

near_r: tyr-1(2 to 4%);Linked to vel (1%),

neu_name:

supplement: phe

image_url:

Strains

FGSC #	Reporting Genes	Species
FGSC #870	T(VI->[I;III])Y16329,phe-2	Neurospora crassa
FGSC #873	phe-2	Neurospora crassa
FGSC #1332	phe-2	Neurospora crassa
FGSC #1333	phe-2	Neurospora crassa
FGSC #3255	phe-2 tyr-1;trp-2	Neurospora crassa
FGSC #4538	sk-2K phe-2 dow (Sk from N. intermedia)	Neurospora crassa
FGSC #4539	Sk-2K phe-2 dow (Sk from N. intermedia)	Neurospora crassa

References

Authors	Title	Year
Baker, T. I.	Phenylalanine-tyrosine biosynthesis in N. crassa.	1968
Brooks, C. J. , B. G. DeBusk, and A. G. DeBusk.	Cellular compartmentation of aromatic amino acids in N. crassa. II. Synthesis andmisplaced accumulation of phenylalanine in phen-2 auxotrophs.	1973
El-Eryani, A. A.	Genetic control of phenylalanine and tyrosine biosynthesis in Neurospora crassa.	1969
Jensen, R. A. , L. Zamir, M. St. Pierre, N. Patel, and D. L. Peirson.	Isolation and preparation of pretyrosine, accumulated as a dead-endmetabolite by Neurospora crassa.	1977
Perkins, D. D. , and E. G. Barry.	Information on chromosome rearrangements.	1977
Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett.	New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage.	1969