FGSC #3255

Mutant Type

Genus: N

reporting_genes: phe-2 tyr-1;trp-2

species: Neurospora crassa

allele: E5212 C167;75001

stock:

glasgow:

mutagen:

Depositor: RLM

Link Group: IIIR IIIR;VIR

MT: a

Species No: 10

gene_back:

oppmt: 0

trans:

ref1: Metzenberg & Mitchell Arch Biochem Biophy 64:51-56 (1956) M77, https://doi.org/10.1016/0003-9861(56)90240-5

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-3255

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-3255 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
trp-2	VIR. Right of del (0 to 13%). Left of un-23 (5 to 27%), T(OY320), and ws-1 (38%) (818, 822, 1019, PB). Uses kynurenine, anthranilic acid, indole, or tryptophan (96). Kynurenine is utilized by conversion to anthranilate (447). Inferred to be the structural gene for the alpha subunit of the anthranilate synthetase complex (546). The gene product catalyzes anthranilate synthesis with ammonia but not with glutamine as the amino donor (29). Specifies anthranitate synthetase (glutamine linked) in collaboration with trp-1 in trifunctional trp-1+-trp-2+ enzyme aggregate (181, 259) (Fig. 11); see trp-1. Nonsense allele used to isolate supersuppressors (954) and to study enzyme complex restored by supersuppressors (183).	VIR	B
tyr-1	IIIR. Right of vel (3 to 5%) and phe-2 (2 to 4%). Left of un-17 (4%) (316, 816, PB; R.L. Metzenberg, personal communication). (47). Requires tyrosine (1055). Lacks prephenate dehydrogenase activity (40, 316) (Fig. 11). Shows phenotypic adaptation after a lag, attaining the wild-type growth rate on minimal medium. Adaptation is not carried through conidia (1055). Allele UT145 formerly called tyr-3 (316).	IIIR	B
phe-2	IIIR. Linked to vel (1%), between T(D305) and tyr-1(2 to 4%) (40, 316, 809). Lacks prephenic dehydratase (40, 316) (Fig. 11). Requirement is very leaky. Grows extensively and is treacherous to score by growth on minimal versus supplemented medium, but can be scored reliably by blue fluorescence under long-wave UV after growth on minimal medium without phenylalanine (816). Appearance of phenylalanine in culture medium (118) is due to spontaneous conversion of accumulated pretyrosine (519). Called phen-2. Allele Y16329 formerly called phen-3 (316).	IIIR	B