Methods described in the Fungal Genetics Newsletter
- Nucleic Acids Extractions
- Alternative medium to simplify DNA extraction
- Fast DNA extraction from Neurospora
- Total nucleic acids from Aspergillus
- Small-scale DNA prep from Neurospora
- DNA for PCR from Fusarium
- Total DNA from filamentous fungi
- Quick RNA miniprep from Neurospora(ATA)
- RNA miniprep from Neurospora(Phenol,LiCl)
- DNA extraction from Coccidioides
- Phenol/ChCl3 based DNA extraction from Aspergillus
- Isolation of nuclei
- Cloning tools and Library manipulations
- Walking in the pMOcosX library
- Vectors for Hygromycin or Phleomycin
- Vectors for expression of cDNA
- Improved vectors for Hygromycin selection
- Working with the Neurospora Yac library
- 6 compact vectors for fungal transformation
- Lambda IIBargem7B
- A hybrid vector supporting large inserts
- Transformation
- Magnaporthe transformation using phosphinothricin selection
- DNA structure and its effect on transformation
- Use of bialophos as a selectable marker
- Protoplasting different cell types of Neurospora
- Use of lipofectin and its effect on transformation
- PCR
- PCR from spores
- Quick PCR from Coprinus
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