FGSC #176

Mutant Type

Genus: N

reporting_genes: Not available; T(I;IV)HK53 cut mei-1

species: Neurospora crassa

allele: HK53

stock: 1276T, ATCC 28607

glasgow:

mutagen:

Depositor: DDP

Link Group: IC;IVC

MT: A

Species No: 10

gene_back:

oppmt: 0

trans:

ref1: Kuwana, H. Jap. J. Genetics 35:49-57 1960 K83, https://doi.org/10.1266/jjg.36.187

ref2: Kuwana 1953. Cytologia 18:235-239, https://doi.org/10.1508/cytologia.18.235

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-176

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-176 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
mei-1	IVR. Linked to arg-2 (<1%), probably to the right (995). Meiosis is impaired in homozygous crosses. Recessive. Meiotic divisions occur and many ascospores are produced, but 70 to 90% are inviable and white. The viable ascospores are usually disomic for one or more linkage groups, indicating high nondisjunction at the first division (254, 995). Chromosome pairing is defective: axial elements of synaptonemal complex are present, but a completed complex is rarely seen. Separation at anaphases I and II is defective, leading to four-poled second and third division spindles (625). Not sensitive to UV, methyl methane sulfonate, ionizing radiation (939), or histidine (939; D. Newmeyer, unpublished data). mei-1 is present in wild-collected strain Abbott 4A (995), which is an ancestor of many Beadle and Tatum mutants (68). Possible allele asc(DL243)complements mei-1 but did not recombine with it (0/3,000) (253). DL243 and mei-1 strains differ phenotypically. In DL243 mutants, the major block is before karyogamy; the few asci produced have normal meiosis I but high nondisjunction at meiosis II, with most chromosomes usually attached to only one spindle-pole body (254). Possible allele asc(DL95) complements mei-1 and asc(DL243), but did not recombine with DL243 (0/96) (253). DL95 is phenotypically like mei-1 but is less extreme (254).	IVR	B
T(I;IV)HK53	Translocation		B
cut	IVL. Between cys-10 (28 to 37%) and fi (4 to 10%) (802; D.D. Perkins, unpublished data). Linkage to IR, shown in original cut strain HK53, was due to an unrecognized I;IV translocation (808). When an allelic cut point mutation (LLMI) became available, it was mapped in IV rather than in I and segregated independently of I markers (802). Sensitive to high osmotic pressure. Phenotype similar to that of os mutants. Scorable either by morphology or by failure to grow on agar containing 4% NaCl. Morphology approaches normal at high humidity (573). Allele HK53 is inseparable from T(I;IVL)HK53 (808).	IVL	B