FGSC #9449

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Reporting Genes: col-16 gna-1

Species: crassa

Allele: R2539, ?

Alternate Strain Number: OG84

Depositor: OG

Linkage Group: IIIR

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
col-16	IIIR. Linked to leu-1 (1%) and pro-1 (10%) (382; PB).Colonial morphology (382). Forms balls of powdery conidia at top of slants of glycerol complete medium. A good marker, preferable to com; strains carrying the latter grow more slowly and do not conidiate (PB). Complements mo-4 and spco-15 (382).	IIIR	B
gna-1	IIIR. Linked to ro-2 (0/17). Encodes guanine nucleotide-binding protein alpha-1-subunit. The predicted amino acid sequence shows significant homology to the Gi family found in higher organisms. The null mutant shows female infertility, osmotic sensitivity, reduced hyphal growth rate, and defective macroconidiation, has decreased intracellular cAMP levels, and is more resistant to some environmental stresses than wild type. Mutants that are predicted to maintain an activated state of GNA-1 have increased intracellular cAMP levels, overproduce aerial hyphae, and are more sensitive to environmental stresses than wild type. Null-mutant extracts have reduced Mg^2+ -dependent adenylyl cyclase and cAMP phosphodiesterase activity. Mg^2+ -dependent adenylyl cyclase activity in wild-type extracts can be inhibited by using antibody against GNA-1.	IIIR	B